Exo-1,4-β-xylosidase
Exo-1,4-β-xylosidase structure
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Common Name | Exo-1,4-β-xylosidase | ||
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| CAS Number | 9025-53-0 | Molecular Weight | 188.146 | |
| Density | 1.2±0.1 g/cm3 | Boiling Point | 194.5±35.0 °C at 760 mmHg | |
| Molecular Formula | C9H7F3O | Melting Point | N/A | |
| MSDS | USA | Flash Point | 78.1±17.4 °C | |
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A novel xylan degrading β-D-xylosidase: purification and biochemical characterization.
World J. Microbiol. Biotechnol. 28(11) , 3179-86, (2012) Aspergillus ochraceus, a thermotolerant fungus isolated in Brazil from decomposing materials, produced an extracellular β-xylosidase that was purified using DEAE-cellulose ion exchange chromatography, Sephadex G-100 and Biogel P-60 gel filtration. β-xylosidas... |
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Beta-xylosidase activity of a GH3 glucosidase/xylosidase from yak rumen metagenome promotes the enzymatic degradation of hemicellulosic xylans.
Lett. Appl. Microbiol. 54(2) , 79-87, (2012) To characterize the duel activities of a glycosyl hydrolase family 3 β-glucosidase/xylosidase from rumen bacterial metagenome and to investigate the capabilities of its β-d-xylosidase activities for saccharification of hemicellulosic xylans. A β-glucosidas... |
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Purification and characterization of a recombinant β-D-xylosidase from Thermobifida fusca TM51.
Protein J. 31(8) , 641-50, (2012) The subject of our investigations was a recombinant β-D-xylosidase (TfBXyl43) from Thermobifida fusca TM51 which was expressed in E. coli BL21DE3 and was purified to apparent homogeneity. The SDS-PAGE investigations demonstrated that the molecular weight of t... |
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Biochemical and kinetic characterization of GH43 β-D-xylosidase/α-L-arabinofuranosidase and GH30 α-L-arabinofuranosidase/β-D -xylosidase from rumen metagenome.
J. Ind. Microbiol. Biotechnol. 39(1) , 143-52, (2012) The present study focuses on characterization of two hemicellulases, RuXyn1 and RuXyn2, from rumen bacterial metagenome and their capabilities for degradation of xylans. Glycosyl hydrolase (GH) family 43 β-D -xylosidase/α-L -arabinofuranosidase RuXyn1 can hyd... |
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Two xylose-tolerant GH43 bifunctional β-xylosidase/α-arabinosidases and one GH11 xylanase from Humicola insolens and their synergy in the degradation of xylan.
Food Chem. 148 , 381-7, (2014) Two β-xylosidases of family 43 (Xyl43A and Xyl43B) and one xylanase of family 11 (Xyn11A) were identified from the genome sequence of Humicola insolens Y1, and their gene products were successfully expressed in heterologous hosts. The optimal activities of th... |
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Activation of a GH43 β-xylosidase by divalent metal cations: slow binding of divalent metal and high substrate specificity.
Arch. Biochem. Biophys. 533(1-2) , 79-87, (2013) RS223-BX of glycoside hydrolase family 43 is a β-d-xylosidase that is strongly activated (k(cat)/K(m) as much as 116-fold) by the addition of divalent metal cations, Ca(2+), Co(2+), Fe(2+), Mg(2+), Mn(2+) and Ni(2+). Slow activation by Mg(2+) was demonstrated... |
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High-level production of thermotolerant β-xylosidase of Aspergillus sp. BCC125 in Pichia pastoris: characterization and its application in ethanol production.
Bioresour. Technol. 132 , 410-3, (2013) A gene coding for thermotolerant β-xylosidase from Aspergillus sp. BCC125 was characterized. The recombinant enzyme was expressed in methylotrophic yeast Pichia pastoris KM71 and especially high yield of secreted enzyme was obtained. β-xylosidase possessed hi... |
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Opposing influences by subsite − 1 and subsite + 1 residues on relative xylopyranosidase/arabinofuranosidase activities of bifunctional β-D-xylosidase/α-L-arabinofuranosidase
Biochim. Biophys. Acta 1814(12) , 1648-57, (2011) Conformational inversion occurs 7–8 kcal/mol more readily in furanoses than pyranoses. This difference is exploited here to probe for active-site residues involved in distorting pyranosyl substrate toward reactivity. Spontaneous glycoside hydrolysis rates are... |
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The cloning, expression, purification, characterization and modeled structure of Caulobacter crescentus β-Xylosidase I.
World J. Microbiol. Biotechnol. 28(9) , 2879-88, (2012) The xynB1 gene (CCNA 01040) of Caulobacter crescentus that encodes a bifunctional enzyme containing the conserved β-Xylosidase and α-L-Arabinofuranosidase (β-Xyl I-α-L-Ara) domains was amplified by PCR and cloned into the vector pJet1.2Blunt. The xynB1 gene w... |
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Penicillium purpurogenum produces two GH family 43 enzymes with β-xylosidase activity, one monofunctional and the other bifunctional: Biochemical and structural analyses explain the difference.
Arch. Biochem. Biophys. 540(1-2) , 117-24, (2013) β-Xylosidases participate in xylan biodegradation, liberating xylose from the non-reducing end of xylooligosaccharides. The fungus Penicillium purpurogenum secretes two enzymes with β-D-xylosidase activity belonging to family 43 of the glycosyl hydrolases. On... |