Bioresource Technology 2013-03-01

High-level production of thermotolerant β-xylosidase of Aspergillus sp. BCC125 in Pichia pastoris: characterization and its application in ethanol production.

Sriwan Wongwisansri, Peerada Promdonkoy, Panida Matetaviparee, Niran Roongsawang, Lily Eurwilaichitr, Sutipa Tanapongpipat

Index: Bioresour. Technol. 132 , 410-3, (2013)

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Abstract

A gene coding for thermotolerant β-xylosidase from Aspergillus sp. BCC125 was characterized. The recombinant enzyme was expressed in methylotrophic yeast Pichia pastoris KM71 and especially high yield of secreted enzyme was obtained. β-xylosidase possessed high enzyme efficiency (Kcat/Km=198.8mM(-1)s(-1)) toward pNP-β-D-xylopyranoside (pNPβX) with optimal temperature and pH for activity of 60°C and pH 4.0-5.0, respectively. The identified β-xylosidase showed clear synergism with previously identified xylanase for hydrolysis of xylan in vitro as well as simultaneous saccharification and fermentation process (SSF) in vivo with Pichia stipitis.Copyright © 2012 Elsevier Ltd. All rights reserved.