Sephacryl
Sephacryl structure
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Common Name | Sephacryl | ||
|---|---|---|---|---|
| CAS Number | 65546-95-4 | Molecular Weight | N/A | |
| Density | N/A | Boiling Point | N/A | |
| Molecular Formula | N/A | Melting Point | N/A | |
| MSDS | N/A | Flash Point | 14 °C | |
| Symbol |
GHS02 |
Signal Word | Warning | |
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Identification of a new alpha-2-macroglobulin: Multi-spectroscopic and isothermal titration calorimetry study.
Int. J. Biol. Macromol. 83 , 366-75, (2016) A α2M homologue was isolated from sheep (Ovis aries) blood plasma, using a simple two-step procedure, ammonium sulphate fractionation and gel filtration chromatography. Sheep α2M was found to be a large tetrameric glycoprotein of 630 kDa with monomeric subuni... |
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Cholesterol stimulates and ceramide inhibits Sticholysin II-induced pore formation in complex bilayer membranes.
Biochim. Biophys. Acta 1848(4) , 925-31, (2015) The pore forming capacity of Sticholysin II (StnII; isolated from Stichodactyla helianthus) in bilayer membranes containing 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), palmitoylsphingomyelin (PSM) and either cholesterol or palmitoyl ceramide (PCe... |
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Expression and purification of cellulase Xf818 from Xylella fastidiosa in Escherichia coli.
Curr. Microbiol. 53(3) , 198-203, (2006) Xylella fastidiosa was the first plant pathogen whose complete genome sequence was available. X. fastidiosa causes citrus variegated chlorosis, but the physiological basis of the disease in unknown. Through comparative sequence analysis, several putative plan... |
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Rat lung possesses the mineralocorticoid receptor.
Biochem. Biophys. Res. Commun. 183(2) , 405-410, (1992) Lung cytosol from male, adrenalectomized rats was screened for the mineralocorticoid receptor (MCR) by a polyclonal antiserum raised in the rabbit against rat renal antigen. Western blot analysis revealed a single 98 kDa band, like the MCR purified biochemica... |
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Heterogeneity of pig lysosomal acid alpha-glucosidase. Affinity to Sephacryl S-200 gel and tissue distribution.
Biochem. J. 279 ( Pt 3) , 719-26, (1991) Acid alpha-glucosidase purified from pig liver showed heterogeneity in its affinity to Sephacryl S-200 gel. Acid alpha-glucosidase was separated into two fractions (S1 and S2) by Sephacryl S-200 affinity chromatography. Each fraction contained components at a... |
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Serodiagnosis of human opisthorchiasis using cocktail and electroeluted Bithynia snail antigens.
Parasitol. Int. 51(3) , 237-47, (2002) Cocktail and electroeluted antigens from Bithynia goniomphalos, the snail intermediate host of Opisthorchis viverrini, were extracted and purified. The performance of these two antigens in the antibody detection of human opisthorchiasis was evaluated by indir... |
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Purification of monoclonal antibodies raised against prostate-specific acid phosphatase for use in vivo in radioimaging of prostatic cancer.
J. Immunol. Methods 117(1) , 131-6, (1989) In developing diagnostic reagents for the radioimaging of prostatic cancer, methods were optimized for the purification of two mouse IgG1 monoclonal antibodies raised against prostate-specific acid phosphatase and produced in cell culture. Two different two-s... |
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Conformational destabilization of Bacillus licheniformis α-amylase induced by lysine modification and calcium depletion.
Acta Biochim. Pol. 58(3) , 405-12, (2011) Bacillus licheniformis α-amylase (BLA) was chemically modified using 100-fold molar excess of succinic anhydride over protein or 0.66 M potassium cyanate to obtain 42 % succinylated and 81 % carbamylated BLAs. Size and charge homogeneity of modified preparati... |
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Caprine (Capra hircus) luteinizing hormone: purification and chromatographic investigation of its different isoforms.
Prep Biochem Biotechnol. 37(3) , 277-300, (2007) Luteinizing Hormone (LH) from goat pituitary glands has been purified and characterized with respect to its size and subunit nature. The purification at each step was monitored by protein estimation, SDS-PAGE, and direct binding ELISA. The final product was f... |
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Development and validation study for the chromatographic purification process for tetanus anatoxin on Sephacryl S-200 High Resolution.
Boll. Chim. Farm. 138(7) , 364-8, (1999) The tetanus purified anatoxin is used in the preparation of multiple immunoprophylactics. WHO (World Health Organization) specifies that the tetanus anatoxin must exhibit a degree of purity greater than or equal to 1,000 Lf/mg protein nitrogen (PN). Today liq... |