Leucine Dehydrogenase
Leucine Dehydrogenase structure
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Common Name | Leucine Dehydrogenase | ||
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| CAS Number | 9082-71-7 | Molecular Weight | N/A | |
| Density | N/A | Boiling Point | N/A | |
| Molecular Formula | N/A | Melting Point | N/A | |
| MSDS | USA | Flash Point | N/A | |
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An efficient and selective enzymatic oxidation system for the synthesis of enantiomerically pure D-tert-leucine.
Org. Lett. 5(20) , 3649-50, (2003) [reaction: see text] d-tert-Leucine was prepared with an enantiomeric excess of >99% by an enzyme-catalyzed oxidative resolution of the racemic mixture of dl-tert-leucine with use of leucine dehydrogenase. The l-amino acid was oxidized completely due to coupl... |
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Foot odor due to microbial metabolism and its control.
Can. J. Microbiol. 52(4) , 357-64, (2006) To characterize foot odor, we analyzed its components by sensory tests, isolated microorganisms that produce it, and evaluated the mechanism of the occurrence of foot odor. As a result, foot odor was found to be derived from isovaleric acid, which is produced... |
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Investigation of mathematical methods for efficient optimisation of aqueous two-phase extraction.
J. Chromatogr. B. Biomed. Sci. Appl. 743(1-2) , 21-30, (2000) Mathematical strategies were applied to optimise the extraction of recombinant leucine dehydrogenase from E. coli homogenates and endoglucanase 1 from culture filtrates of Trichoderma reesei in polyethylene glycol-phosphate systems. The goal was to test mathe... |
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Investigating expression systems for the stable large-scale production of recombinant L-leucine-dehydrogenase from Bacillus cereus in Escherichia coli.
Appl. Microbiol. Biotechnol. 53(6) , 668-73, (2000) The established Escherichia coli expression vectors ptrc99a, pKK223-3, pPLlambda, pAsk75, pRA95, and pRA96, which differ in copy number, mode of induction, selection marker, and use of par sequences for stabilization, were investigated for the stable expressi... |
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Production of recombinant L-leucine dehydrogenase from Bacillus cereus in pilot scale using the runaway replication system E. coli[pIET98].
Biotechnol. Bioeng. 68(5) , 557-62, (2000) A method for the production of recombinant L-leucine dehydrogenase from Bacillus cereus in pilot scale is described employing the temperature induced runaway replication vector pIET98 and the Escherichia coli host strain BL21. Fed-batch cultivation using a se... |
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Cloning, sequencing and overexpression of the leucine dehydrogenase gene from Bacillus cereus.
J. Biotechnol. 54(1) , 77-80, (1997) The L-leucine dehydrogenase gene from Bacillus cereus (DSM 626) was cloned from a partial genomic library and sequenced. The open reading frame has 1101 bp and codes for a protein of 39.9 kDa. The deduced amino acid sequence of the LeuDH from B. cereus shares... |
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Construction and physiological studies on a stable bioengineered strain of shengjimycin.
J. Antibiot. 54(1) , 66-73, (2001) Shengjimycin is a group of 4"-acylated spiramycins with 4"-isovalerylspiramycin as the major component, produced by recombinant S. spiramyceticus F21 harboring a 4"-O-acyltransferase gene from S. mycarofaciens 1748. A stable bioengineered strain of Streptomyc... |
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NADH-dependent inhibition of branched-chain fatty acid synthesis in Bacillus subtilis.
Biosci. Biotechnol. Biochem. 62(4) , 622-7, (1998) Addition of NADH to crude but not to pure branched-chain alpha-keto acid decarboxylase decreased the CO2 production from alpha-keto-beta-methylvalerate (KMV) suggesting the presence of an NADH dependent inhibitor in the crude enzyme from Bacillus subtilis. Th... |
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Fundamental differences in bioaffinity of amino acid dehydrogenases for N6- and S6-linked immobilized cofactors using kinetic-based enzyme-capture strategies.
Anal. Biochem. 338(1) , 102-12, (2005) Five different immobilized NAD+ derivatives were employed to compare the behavior of four amino acid dehydrogenases chromatographed using kinetic-based enzyme capture strategies (KBECS): S6-, N6-, N1-, 8'-azo-, and pyrophosphate-linked immobilized NAD+. The a... |
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Synthesis of probes for the active site of leucine dehydrogenase.
Bioorg. Med. Chem. Lett. 9(14) , 1941-4, (1999) A series of novel 3-substituted 2-oxobutanoic acids were prepared and incubated with leucine dehydrogenase giving in one case both a kinetic resolution at C-3 and reductive amination of the ketone. This is the first example of an amino acid dehydrogenase cata... |