Inulinase fromAspergillus niger
Inulinase fromAspergillus niger structure
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Common Name | Inulinase fromAspergillus niger | ||
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| CAS Number | 9025-67-6 | Molecular Weight | 248.298 | |
| Density | 1.3±0.1 g/cm3 | Boiling Point | 425.8±45.0 °C at 760 mmHg | |
| Molecular Formula | C13H12O3S | Melting Point | N/A | |
| MSDS | Chinese USA | Flash Point | 211.3±28.7 °C | |
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Ethanol production from inulin and unsterilized meal of Jerusalem artichoke tubers by Saccharomyces sp. W0 expressing the endo-inulinase gene from Arthrobacter sp.
Bioresour. Technol. 147 , 254-9, (2013) After the endo-inulinase gene from Arthrobacter sp. was ligated the expression vectors pMIDSC31 and pMIRSC31, the endo-inulinase gene was inserted into the chromosomal DNA of Saccharomyces sp. W0. It was found that the inulinase activity of the recombinant ye... |
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Molecular characterization and expression of microbial inulinase genes.
Crit. Rev. Microbiol 39(2) , 152-65, (2013) Many genes encoding exo- and endo-inulinases from bacteria, yeasts and filamentous fungi have been cloned and characterized. All the inulinases have several conserved motifs, such as WMND(E)PNGL, RDP, EC(V)P, SVEVF, Q and FS(T), which play an important role i... |
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First crystal structure of an endo-inulinase, INU2, from Aspergillus ficuum: discovery of an extra-pocket in the catalytic domain responsible for its endo-activity.
Biochimie 94(11) , 2423-30, (2012) Endo-inulinase is a member of glycosidase hydrolase family 32 (GH32) degrading fructans of the inulin type with an endo-cleavage mode and is an important class of industrial enzyme. In the present study, we report the first crystal structure of an endo-inulin... |
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Consolidated bioprocessing of highly concentrated Jerusalem artichoke tubers for simultaneous saccharification and ethanol fermentation.
Biotechnol. Bioeng. 110(10) , 2606-15, (2013) Consolidated bioprocessing (CBP) of Jerusalem artichoke tuber (Jat) for ethanol production is one of the most promising options for an alternate biofuel technology development. The technical barriers include the weak saccharolytic enzyme (inulinase) activity ... |
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Expression of family 3 cellulose-binding module (CBM3) as an affinity tag for recombinant proteins in yeast.
Appl. Microbiol. Biotechnol. 91(3) , 789-98, (2011) Easy and low-cost protein purification methods for the mass production of commonly used enzymes that play important roles in biotechnology are in high demand. In this study, we developed a fast, low-cost recombinant protein purification system in the methylot... |
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Cloning and sequencing of inulinase and β-fructofuranosidase genes of a deep-sea Microbulbifer species and properties of recombinant enzymes.
Appl. Environ. Microbiol. 78(7) , 2493-5, (2012) An inulinase-producing Microbulbifer sp. strain, JAM-3301, was isolated from a deep-sea sediment. An inulin operon that contained three open reading frames was cloned and sequenced. Two of the three genes were expressed. One product was an endo-inulinase, and... |
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Cloning of exoinulinase gene from Penicillium janthinellum strain B01 and its high-level expression in Pichia pastoris.
J. Appl. Microbiol. 111(6) , 1371-80, (2011) The aim of this study is to improve exoinulinase production by expression of a cloned exoinulinase gene inuA1 (GenBank accession no. JF961344) from Penicillium janthinellum strain B01 in Pichia pastoris.A full-length cDNA of exoinulinase gene (inuA1) was clon... |
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Expression of an exoinulinase gene from Aspergillus ficuum in Escherichia coli and its characterization.
Carbohydr. Polym. 92(2) , 1984-90, (2013) An exoinulinase gene from Aspergillus ficuum JNSP5-06 was overexpressed in Escherichia coli. Two exons of the exoinulinase gene were amplified separately, joined together by an overlap PCR, and expressed in E. coli. The molecular weight of the recombinant exo... |
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Application of low magnetic field on inulinase production by Geotrichum candidum under solid state fermentation using leek as substrate.
Toxicol. Ind. Health 28(10) , 894-900, (2012) This study evaluates the application of low magnetic field (LMF) on inulinase enzyme production by Geotrichum candidum under solid state fermentation (SSF) using leek as potential carbon source. First, the fermentation conditions were optimized using normal m... |
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Cloning, characterization and heterelogous expression of the INU1 gene from Cryptococcus aureus HYA.
Gene 516(2) , 255-62, (2013) The INU1 gene (Accession number: JX073660) encoding exo-inulinase from Cryptococcus aureus HYA was cloned and characterized. The gene had an open reading frame (ORF) of 1653 bp long encoding an inulinase. The coding region of the gene was not interrupted by a... |