ddGTP
ddGTP结构式
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常用名 | ddGTP | 英文名 | ddGTP |
|---|---|---|---|---|
| CAS号 | 68726-28-3 | 分子量 | 491.18200 | |
| 密度 | N/A | 沸点 | N/A | |
| 分子式 | C10H16N5O12P3 | 熔点 | N/A | |
| MSDS | 美版 | 闪点 | N/A |
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Selective enzymatic cleavage and labeling for sensitive capillary electrophoresis laser-induced fluorescence analysis of oxidized DNA bases.
J. Chromatogr. A. 1406 , 324-30, (2015) Oxidatively generated DNA damage is considered to be a significant contributing factor to cancer, aging, and age-related human diseases. It is important to detect oxidatively generated DNA damage to understand and clinically diagnosis diseases caused by oxida... |
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Single methylation of 23S rRNA triggers late steps of 50S ribosomal subunit assembly.
Proc. Natl. Acad. Sci. U. S. A. 112 , E4707-16, (2015) Ribosome biogenesis requires multiple assembly factors. In Escherichia coli, deletion of RlmE, the methyltransferase responsible for the 2'-O-methyluridine modification at position 2552 (Um2552) in helix 92 of the 23S rRNA, results in slow growth and accumula... |
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DNA sequencing with chain-terminating inhibitors.
Proc. Natl. Acad. Sci. U. S. A. 74 , 5463, (1977) A new method for determining nucleotide sequences in DNA is described. It is similar to the "plus and minus" method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2',3'-dideoxy and arabinonucleoside analogues of the normal... |
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Interactions of tubulin with guanine nucleotides that have paclitaxel-like effects on tubulin assembly: 2',3'-dideoxyguanosine 5'-[alpha,beta-methylene]triphosphate, guanosine 5'-[alpha,beta-methylene]triphosphate, and 2',3'-dideoxyguanosine 5'-triphosphate.
Arch. Biochem. Biophys. 322(2) , 486-99, (1995) Despite reduced affinity for the exchangeable nucleotide binding site of tubulin relative to GTP, 2',3'-dideoxyguanosine 5'-triphosphate (ddGTP) and guanosine 5'-[alpha, beta-methylene]triphosphate [pp(CH2)pG] are highly active in promoting tubulin assembly. ... |
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A primer extension method for quantitation of in vitro synthesized mRNA transcripts directed by promoter elements containing multiple transcription initiation sites.
Anal. Biochem. 232(2) , 258-61, (1995)
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A new randomization assay reveals unexpected elements of sequence bias in model 'randomized' gene libraries: implications for biopanning.
Gene 251(1) , 91-9, (2000) Although techniques such as biopanning rely heavily upon the screening of randomized gene libraries, there is surprisingly little information available on the construction of those libraries. In general, it is based on the cloning of 'randomized' synthetic ol... |
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Direct detection of methylation in genomic DNA.
Nucleic Acids Res. 33(14) , e124, (2005) The identification of methylated sites on bacterial genomic DNA would be a useful tool to study the major roles of DNA methylation in prokaryotes: distinction of self and nonself DNA, direction of post-replicative mismatch repair, control of DNA replication a... |
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Enzymatic properties and sensitivity to inhibitors of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase with Glu-138-->Arg and Tyr-188-->His mutations.
Antiviral Res. 24(1) , 43-57, (1994) Two mutants of HIV-1 reverse transcriptase (RT), Tyr-188-->His and Glu-138-->Arg have been prepared and their catalytic properties and sensitivities to inhibitors studied. As compared to wild type RT, a reduction in catalytic efficiency and turn over number w... |
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Real-time single-molecule observation of rolling-circle DNA replication.
Nucleic Acids Res. 37(4) , e27, (2009) We present a simple technique for visualizing replication of individual DNA molecules in real time. By attaching a rolling-circle substrate to a TIRF microscope-mounted flow chamber, we are able to monitor the progression of single-DNA synthesis events and ac... |
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A two plasmid co-expression system in Escherichia coli for the production of virion-like reverse transcriptase of the human immunodeficiency virus type 1.
J. Virol. Methods 61(1-2) , 113-25, (1996) Many bacterial expression systems have been developed to study the reverse transcriptase (RT) of human immunodeficiency virus type 1 (HIV-1). This enzyme exists in the virions as a heterodimer of a 66 kDa (p66) subunit and a 51 kDa (p51) subunit, originating ... |