试卤灵Β-D-半乳糖吡喃糖苷
试卤灵Β-D-半乳糖吡喃糖苷结构式
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常用名 | 试卤灵Β-D-半乳糖吡喃糖苷 | 英文名 | Resorufin β-D-Galactopyranoside |
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| CAS号 | 95079-19-9 | 分子量 | 375.32900 | |
| 密度 | 1.71 g/cm3 | 沸点 | 651.6ºCat 760 mmHg | |
| 分子式 | C18H17NO8 | 熔点 | N/A | |
| MSDS | 美版 | 闪点 | 347.9ºC |
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A single-cell assay of beta-galactosidase activity in Saccharomyces cerevisiae.
Cytometry 9 , 394, (1988) A novel assay of single-cell exogenous beta-galactosidase activity in Saccharomyces cerevisiae has been developed. Intracellular fluorescence due to the hydrolysis of resorufin-beta-D-galactopyranoside attains a steady state between production of resorufin an... |
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Ever-fluctuating single enzyme molecules: Michaelis-Menten equation revisited.
Nat. Chem. Biol. 2(2) , 87-94, (2006) Enzymes are biological catalysts vital to life processes and have attracted century-long investigation. The classic Michaelis-Menten mechanism provides a highly satisfactory description of catalytic activities for large ensembles of enzyme molecules. Here we ... |
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Single-shot characterization of enzymatic reaction constants Km and kcat by an acoustic-driven, bubble-based fast micromixer.
Anal. Chem. 84(17) , 7495-501, (2012) In this work we present an acoustofluidic approach for rapid, single-shot characterization of enzymatic reaction constants K(m) and k(cat). The acoustofluidic design involves a bubble anchored in a horseshoe structure which can be stimulated by a piezoelectri... |
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Determination of kinetic parameters, Km and kcat, with a single experiment on a chip.
Anal. Chem. 81(9) , 3239-45, (2009) We have demonstrated a multistep enzyme reaction on a chip to determine the key kinetic parameters of enzyme reaction. We designed and fabricated a fully integrated microfluidic chip to have sample metering, mixing, and incubation functionalities. The chip ge... |
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Fast mixing and reaction initiation control of single-enzyme kinetics in confined volumes.
Langmuir 24(9) , 4439-42, (2008) A device with femtoliter-scale chambers and controlled reaction initiation was developed for single-molecule enzymology. Initially separated substrate and enzyme streams were rapidly mixed in a microfluidic device and encapsulated in an array of individual mi... |
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Crystallization of beta-galactosidase does not reduce the range of activity of individual molecules.
Biochemistry 42(6) , 1707-10, (2003) By use of a capillary electrophoresis-based procedure, it is possible to measure the activity of individual molecules of beta-galactosidase. Molecules from the crystallized enzyme as well as the original enzyme preparation used to grow the crystals both displ... |
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beta-galactosidase assay using capillary electrophoresis laser-induced fluorescence detection and resorufin-beta-D-galactopyranoside as substrate.
Biomed. Chromatogr. 13(8) , 516-9, (1999) beta-Galactosidase was incubated for 60 min with the fluorogenic substrate resorufin-beta-D-galactopyranoside, which is converted by the action of the enzyme into resorufin and galactose. A 160 pL aliquot of reaction mixture was analyzed by capillary electrop... |