多核苷酸磷酸化酶
多核苷酸磷酸化酶结构式
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常用名 | 多核苷酸磷酸化酶 | 英文名 | POLYNUCLEOTIDE PHOSPHORYLASE FROM BACIL |
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| CAS号 | 9014-12-4 | 分子量 | N/A | |
| 密度 | N/A | 沸点 | N/A | |
| 分子式 | N/A | 熔点 | N/A | |
| MSDS | N/A | 闪点 | N/A |
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Discrimination of RNA from DNA by polynucleotide phosphorylase.
Biochemistry 52(38) , 6702-11, (2013) Polynucleotide phosphorylase (PNPase) plays synthetic and degradative roles in bacterial RNA metabolism; it is also thought to participate in bacterial DNA transactions. Here we used chimeric polynucleotides, composed of alternating RNA and DNA tracts, to ana... |
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Streptomyces coelicolor polynucleotide phosphorylase can polymerize nucleoside diphosphates under phosphorolysis conditions, with implications for the degradation of structured RNAs.
J. Bacteriol. 195(22) , 5151-9, (2013) We have examined the ability of wild-type polynucleotide phosphorylase (PNPase) from Streptomyces coelicolor and two mutant forms of the enzyme, N459D and C468A, to function in the polymerization of ADP and in the phosphorolysis of RNA substrates derived from... |
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Distinctive effects of domain deletions on the manganese-dependent DNA polymerase and DNA phosphorylase activities of Mycobacterium smegmatis polynucleotide phosphorylase.
Biochemistry 52(17) , 2967-81, (2013) Polynucleotide phosphorylase (PNPase) plays synthetic and degradative roles in bacterial RNA metabolism; it is also suggested to participate in bacterial DNA transactions. Here we characterize and compare the RNA and DNA modifying activities of Mycobacterium ... |
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Human mitochondrial RNA decay mediated by PNPase-hSuv3 complex takes place in distinct foci.
Nucleic Acids Res. 41(2) , 1223-40, (2013) RNA decay is usually mediated by protein complexes and can occur in specific foci such as P-bodies in the cytoplasm of eukaryotes. In human mitochondria nothing is known about the spatial organization of the RNA decay machinery, and the ribonuclease responsib... |
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DEAD-Box RNA Helicases in Gram-Positive RNA Decay-Chapter Seventeen
Meth. Enzymol. 511 , 369-83, (2012) DEAD-box RNA helicases are important players in eukaryotic and bacterial RNA metabolism. A helicase from Staphylococcus aureus was recently shown to affect RNA decay, most likely via its interaction with the proposed Gram-positive degradosome. Some, but not a... |
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An RNA degradation machine sculpted by Ro autoantigen and noncoding RNA.
Cell 153(1) , 166-77, (2013) Many bacteria contain an ortholog of the Ro autoantigen, a ring-shaped protein that binds noncoding RNAs (ncRNAs) called Y RNAs. In the only studied bacterium, Deinococcus radiodurans, the Ro ortholog Rsr functions in heat-stress-induced ribosomal RNA (rRNA) ... |
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CvfA protein and polynucleotide phosphorylase act in an opposing manner to regulate Staphylococcus aureus virulence.
J. Biol. Chem. 289(12) , 8420-31, (2014) We previously identified CvfA (SA1129) as a Staphylococcus aureus virulence factor using a silkworm infection model. S. aureus cvfA-deleted mutants exhibit decreased expression of the agr locus encoding a positive regulator of hemolysin genes and decreased he... |
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Recognition of the 70S ribosome and polysome by the RNA degradosome in Escherichia coli.
Nucleic Acids Res. 40(20) , 10417-31, (2012) The RNA degradosome is a multi-enzyme assembly that contributes to key processes of RNA metabolism, and it engages numerous partners in serving its varied functional roles. Small domains within the assembly recognize collectively a diverse range of macromolec... |
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Characterization of small RNAs and their target genes in wheat seedlings using sequencing-based approaches.
Plant Sci. 203-204 , 17-24, (2013) Wheat is the most highly cultivated plant species for its grain production throughout the world. Because small RNA-dependent gene regulation is critical for successful completion of plant life cycle including its productivity, identification of not only miRNA... |
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Bacterial small RNA-based negative regulation: Hfq and its accomplices.
J. Biol. Chem. 288(12) , 7996-8003, (2013) A large group of bacterial small regulatory RNAs (sRNAs) use the Hfq chaperone to mediate pairing with and regulation of mRNAs. Recent findings help to clarify how Hfq acts and highlight the role of the endonuclease RNase E and its associated proteins (the de... |