Ficoll®,70型
Ficoll®,70型结构式
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常用名 | Ficoll®,70型 | 英文名 | FicollPM 70 |
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| CAS号 | 72146-89-5 | 分子量 | N/A | |
| 密度 | N/A | 沸点 | N/A | |
| 分子式 | N/A | 熔点 | N/A | |
| MSDS | 美版 | 闪点 | N/A |
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Protein disorder prevails under crowded conditions.
Biochemistry 50 , 5834-5844, (2011) Crowding caused by the high concentrations of macromolecules in the living cell changes chemical equilibria, thus promoting aggregation and folding reactions of proteins. The possible magnitude of this effect is particularly important with respect to the phys... |
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Cell-free protein expression under macromolecular crowding conditions.
PLoS ONE 6 , e28707, (2011) Cell-free protein expression (CFPE) comprised of in vitro transcription and translation is currently manipulated in relatively dilute solutions, in which the macromolecular crowding effects present in living cells are largely ignored. This may not only affect... |
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Light scattering studies on Ficoll PM70 solutions reveal two distinct diffusive modes.
J. Colloid. Interface Sci. 386(1) , 141-7, (2012) Ficoll PM70 is an important branched polysaccharide polymer with a variety of applications in biology and biophysics. The accepted picture for the aqueous solutions of this polymer is that of a polydisperse nearly spherical suspension with a molecular weight ... |
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Protein folding by the effects of macromolecular crowding.
Protein Sci. 13 , 125-133, (2004) Unfolded states of ribonuclease A were used to investigate the effects of macromolecular crowding on macromolecular compactness and protein folding. The extent of protein folding and compactness were measured by circular dichroism spectroscopy, fluorescence c... |
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Macromolecular crowding as a cell volume sensor.
Cell Physiol. Biochem. 10 , 251-256, (2000) The non-ideal properties of solutions containing high concentrations of macromolecules can result in enormous increases in the activity of the individual macromolecules. There is considerable evidence that macromolecular crowding and confinement not only occu... |
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Molecular crowding reduces to a similar extent the diffusion of small solutes and macromolecules: measurement by fluorescence correlation spectroscopy.
J. Mol. Recognit. 17(5) , 441-7, (2004) Aqueous environments in living cells are crowded, with up to >50 wt% small and macromolecule-size solutes. We investigated quantitatively one important consequence of molecular crowding--reduced diffusion of biologically important solutes. Fluorescence correl... |
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Macromolecular crowding and its role as intracellular signalling of cell volume regulation.
Int. J. Biochem. Cell Biol. 33 , 844-864, (2001) Macromolecular crowding has been proposed as a mechanism by means of which a cell can sense relatively small changes in volume or, more accurately, the concentration of intracellular solutes. According to the macromolecular theory, the kinetics and equilibria... |