琼脂糖 CL-6B
琼脂糖 CL-6B结构式
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常用名 | 琼脂糖 CL-6B | 英文名 | SepharoseCL-6B |
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| CAS号 | 62610-50-8 | 分子量 | N/A | |
| 密度 | 1.040 | 沸点 | 100℃ | |
| 分子式 | N/A | 熔点 | N/A | |
| MSDS | 美版 | 闪点 | 39 °C |
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Integrated system for temperature-controlled fast protein liquid chromatography comprising improved copolymer modified beaded agarose adsorbents and a travelling cooling zone reactor arrangement.
J. Chromatogr. A. 1285 , 97-109, (2013) An integrated approach to temperature-controlled chromatography, involving copolymer modified agarose adsorbents and a novel travelling cooling zone reactor (TCZR) arrangement, is described. Sepharose CL6B was transformed into a thermoresponsive cation exchan... |
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Studies on the retention of plasmid DNA and Escherichia coli nucleic acids by hydrophobic interaction chromatography.
Bioseparation 10(4-5) , 211-20, (2001) This work presents studies on the interactions of supercoiled plasmid DNA and Escherichia coli genomic DNA (gDNA) and RNA, with an hydrophobic interaction chromatography (HIC) gel, obtained by derivatisation of Sepharose CL-6B with 1,4-butanediol diglycidyl e... |
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Structural and catalytic properties of the D-3-hydroxybutyrate dehydrogenase from Pseudomonas aeruginosa.
Curr. Microbiol. 61 , 7-12, (2010) To put forward BDH from Pseudomonas aeruginosa's enzymatic properties, we report a two-step purification of BDH and its gene sequencing allowing the investigation of its structural properties. Purification of BDH was achieved, using ammonium sulfate fractiona... |
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A cytotoxic type-2 ribosome inactivating protein (from leafless mistletoe) lacking sugar binding activity.
Int. J. Biol. Macromol. 49(5) , 1096-103, (2011) Articulatin-D, a 66 kDa ribosome inactivating protein (RIP) comprised of 29 kDa A-chain linked to 35 kDa B-chain, is purified from leafless mistletoe (Viscum articulatum) parasitic on Dalbergia sp. from Western Ghats (India). N-terminal sequence and LC-MS/MS ... |
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Esterase 2 from Alicyclobacillus acidocaldarius as a reporter and affinity tag for expression and single step purification of polypeptides.
Protein Expr. Purif. 54(1) , 94-100, (2007) A novel dual function (reporter and affinity) tag system has been developed. Expression vectors have been constructed to express polypeptides in Escherichia coli cells as C-terminal fusions with esterase 2, a 34-kDa protein from Alicyclobacillus acidocaldariu... |
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Adsorption of inulinases in ion-exchange columns.
Appl. Biochem. Biotechnol. 84-86 , 1063-78, (2000) The use of adsorption columns packed with ion-exchange resins for recovering, concentrating and purifying proteins is now widespread. The present work consists of a study on the dynamic behavior of adsorption columns that uses two kinds of adsorbents: a catio... |
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Purification and characterization of an immunomodulatory Peptide from bovine placenta water-soluble extract.
Prep Biochem Biotechnol. 37(3) , 173-84, (2007) An immunomodulatory peptide was isolated from bovine placenta water-soluble extract and purified by consecutive chromatography on DEAE Sepharose CL-6B, Sephadex G-25, and Sephasil C18 column using lymphocyte proliferation assay to identify the active fraction... |
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Rapid purification of PCR products for DNA sequencing using Sepharose CL-6B spin columns.
Biotechniques 8(3) , 271-4, (1990)
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E. coli penicillin acylase: purification by affinity chromatography and covalent binding to nylon.
Il Farmaco 45(2) , 203-14, (1990) Penicillin acylase (EC 3.5.1.11) from E. coli, both in solution and immobilized on solid supports, has been commercially exploited for the large scale production of 6-aminopenicillanic acid (6-APA), which is an important intermediate for the manufacturing of ... |
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The cluster structure of barley amylopectins of different genetic backgrounds.
Int. J. Biol. Macromol. 49(4) , 441-53, (2011) The unit chains of amylopectin are organized into clusters. In this study, the cluster structure was analysed in detail in four different genotypes of barley, of which two possessed the amo1 genetic background. Amylose content of the barley starches differed ... |