葡聚糖蔗糖酶
葡聚糖蔗糖酶结构式
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常用名 | 葡聚糖蔗糖酶 | 英文名 | Dextran sucrase |
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| CAS号 | 9032-14-8 | 分子量 | 221.209 | |
| 密度 | 1.2±0.1 g/cm3 | 沸点 | 326.6±17.0 °C at 760 mmHg | |
| 分子式 | C11H11NO4 | 熔点 | N/A | |
| MSDS | 美版 | 闪点 | 141.1±22.9 °C |
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Branching pattern of gluco-oligosaccharides and 1.5kDa dextran grafted by the α-1,2 branching sucrase GBD-CD2.
Carbohydr. Polym. 94(1) , 567-76, (2013) GBD-CD2, an engineered sucrose-acting enzyme of glycoside hydrolase family 70, transfers D-glucopyranosyl (D-Glcp) units from sucrose onto dextrans or gluco-oligosaccharides (GOS) through the formation of α-(1→2) linkages leading to branched products of inter... |
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Purification, optimization of assay, and stability studies of dextransucrase isolated from Weissella cibaria JAG8.
Prep Biochem Biotechnol. 43(4) , 329-41, (2013) Dextransucrase-producing (Gen Bank accession no. KC110687) Weissella cibaria JAG8 was isolated from apple. The cell-free extract containing dextransucrase with specific activity of 1.0 U/mg was purified by polyethylene glycol (PEG). A concentration of 33% (v/... |
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Synthesis and characterization of ampelopsin glucosides using dextransucrase from Leuconostoc mesenteroides B-1299CB4: glucosylation enhancing physicochemical properties.
Enzyme Microb. Technol. 51(6-7) , 311-8, (2012) Novel ampelopsin glucosides (AMPLS-Gs) were enzymatically synthesized and purified using a Sephadex LH-20 column. Each structure of the purified AMPLS-Gs was determined by nuclear magnetic resonance, and the ionic product of AMPLS-G1 was observed at m/z 505 (... |
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Inhibition of dextransucrase activity in Streptococcus mutans by plant phenolics.
Indian J. Biochem. Biophys. 50(1) , 48-53, (2013) Streptococcus mutans is responsible for causing dental caries in humans and utilizes sucrose for its growth. The dextransucrase (EC 2.4.1.5) is responsible for sucrose metabolism, which exhibits both hydrolytic and glucosyltransferase activities. In this stud... |
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Enzymatic synthesis of puerarin glucosides using Leuconostoc dextransucrase.
J. Microbiol. Biotechnol. 22(9) , 1224-9, (2012) Puerarin (P), an isoflavone derived from kudzu roots, has strong biological activities, but its bioavailability is often limited by its low water solubility. To increase its solubility, P was glucosylated by three dextransucrases from Leuconostoc or Streptoco... |
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Enzymatic synthesis of alkyl glucosides using Leuconostoc mesenteroides dextransucrase.
Biotechnol. Lett. 31(9) , 1433-8, (2009) Alkyl glucosides were synthesized by the reaction of Leuconostoc mesenteroides dextransucrase with sucrose and various alcohols. Alkyl alpha-D-glucosides were obtained with a yield of 30% (mol/mol) with primary alcohols, but secondary alcohols or tertiary alc... |
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Application of response surface methodology for maximizing dextransucrase production from Leuconostoc mesenteroides NRRL B-640 in a bioreactor.
Appl. Biochem. Biotechnol. 151(2-3) , 182-92, (2008) The production of dextransucrase from Leuconostoc mesenteroides NRRL B-640 was investigated using statistical approaches. Plackett-Burman design with six variables, viz. sucrose, yeast extract, K(2)HPO(4), peptone, beef extract, and Tween 80, was used to scre... |
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Large increase in Leuconostoc citreum KM20 dextransucrase activity achieved by changing the strain/inducer combination in an E. coli expression system.
J. Microbiol. Biotechnol. 22(4) , 510-5, (2012) A recombinant putative dextransucrase (DexT) was produced from Leuconostoc citreum KM20 as a 160 kDa protein, but its productivity was very low (264 U/l). For optimization, we examined enzyme activity in 7 Escherichia coli strains with inducer molecules such ... |
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Monitoring of Leuconostoc population during sauerkraut fermentation by quantitative real-time polymerase chain reaction.
J. Microbiol. Biotechnol. 21(10) , 1069-72, (2011) A real-time PCR assay method was established to monitor Leuconostoc spp. populations via specific amplification of the dextransucrase gene. Quantification of L. mesenteroides B-512F using both genomic DNA and cell suspensions yielded a log-linear correlation ... |
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Development of a high-throughput screening method for recombinant Escherichia coli with intracellular dextransucrase activity.
J. Microbiol. 49(2) , 320-3, (2011) To efficiently engineer intracellular dextransucrase (DSase) expression in Escherichia coli, a high-throughput screening method was developed based on the polymer-forming activity of the enzyme. Recombinant E. coli containing the Leuconostoc citreum DSase (Lc... |