2,3,5-Triphenyltetrazolium chloride

Modify Date: 2025-08-21 19:16:29

2,3,5-Triphenyltetrazolium chloride Structure
2,3,5-Triphenyltetrazolium chloride structure
 Common Name 2,3,5-Triphenyltetrazolium chloride
CAS Number 298-96-4 Molecular Weight 334.802
Density N/A Boiling Point >360
Molecular Formula C19H15ClN4 Melting Point 250 °C(lit.)
MSDS Chinese USA Flash Point 91.7ºC
Symbol GHS07
GHS07		 Signal Word Warning

 Use of 2,3,5-Triphenyltetrazolium chloride


Tetrazolium Red(2,3,5-Triphenyltetrazolium chloride; TPTZ) is used to visualize dehydrogenase enzyme activity; initially the tetrazolium solution is colorless but changes to red when it comes into contact with hydrogen. Tetrazolium red is used in a biochemical viability test for seeds. The test relies on dehydrogenase enzymes to release hydrogen ions which subsequently reduce the colorless tetrazolium salt solution to a red compound called formazan. Living cells turn red while dead cells remain colorless.

 Names

Name 2,3,5-triphenyltetrazolium chloride
Synonym More Synonyms

 2,3,5-Triphenyltetrazolium chloride Biological Activity

Description Tetrazolium Red(2,3,5-Triphenyltetrazolium chloride; TPTZ) is used to visualize dehydrogenase enzyme activity; initially the tetrazolium solution is colorless but changes to red when it comes into contact with hydrogen. Tetrazolium red is used in a biochemical viability test for seeds. The test relies on dehydrogenase enzymes to release hydrogen ions which subsequently reduce the colorless tetrazolium salt solution to a red compound called formazan. Living cells turn red while dead cells remain colorless.
Related Catalog

 Chemical & Physical Properties

Boiling Point >360
Melting Point 250 °C(lit.)
Molecular Formula C19H15ClN4
Molecular Weight 334.802
Flash Point 91.7ºC
Exact Mass 334.098511
PSA 34.59000
LogP 0.21500
Vapour Pressure 0.0615mmHg at 25°C
Storage condition 2-8°C
Stability Stable. Light sensitive. Protect from moisture. Incompatible with strong oxidizing agents.
Water Solubility H2O: may be clear to slightly hazy

 Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :
XF8100000
CHEMICAL NAME :
2H-Tetrazolium, 2,3,5-triphenyl-, chloride
CAS REGISTRY NUMBER :
298-96-4
LAST UPDATED :
199701
DATA ITEMS CITED :
4
MOLECULAR FORMULA :
C19-H15-N4.Cl
MOLECULAR WEIGHT :
334.83
WISWESSER LINE NOTATION :
T5NNKNJ BR& CR& ER &G

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intravenous
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
5600 ug/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
REFERENCE :
CSLNX* U.S. Army Armament Research & Development Command, Chemical Systems Laboratory, NIOSH Exchange Chemicals. (Aberdeen Proving Ground, MD 21010) Volume(issue)/page/year: NX#00925 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - 83592 No. of Facilities: 11 (estimated) No. of Industries: 1 No. of Occupations: 1 No. of Employees: 32 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - 83592 No. of Facilities: 32 (estimated) No. of Industries: 1 No. of Occupations: 2 No. of Employees: 1665 (estimated) No. of Female Employees: 1207 (estimated)

 Safety Information

Symbol GHS07
GHS07
Signal Word Warning
Hazard Statements H315-H319-H335
Precautionary Statements P261-P305 + P351 + P338
Personal Protective Equipment Eyeshields;Gloves
Hazard Codes Xi:Irritant
Risk Phrases R36/37/38
Safety Phrases S26-S36
RIDADR NONH for all modes of transport
WGK Germany 3
RTECS XF8100000
HS Code 2933990090

 Synthetic Route

Diazene, phenyl(phenyl(phenylhydrazono)methyl)- structure

Diazene, phenyl...

CAS#:531-52-2

~95%

2,3,5-Triphenyltetrazolium chloride Structure

2,3,5-Triphenyl...

CAS#:298-96-4

Literature: Balakrishnan, R.; Srinivasan, Vangular S. Indian Journal of Chemistry, Section B: Organic Chemistry Including Medicinal Chemistry, 1983 , vol. 22, # 8 p. 771 - 775
1,3,5-triphenylformazan structure

1,3,5-triphenyl...

CAS#:49624-20-6

~86%

2,3,5-Triphenyltetrazolium chloride Structure

2,3,5-Triphenyl...

CAS#:298-96-4

Literature: Katritzky, Alan R.; Belyakov, Sergey A.; Lam, Jamshed N.; Durst, H. Dupont; Karpenko, Dmitrii V. Heterocycles, 1994 , vol. 39, # 1 p. 73 - 80
t-Butyl hypochlorite structure

t-Butyl hypochlorite

CAS#:507-40-4

Diazene, phenyl(phenyl(phenylhydrazono)methyl)- structure

Diazene, phenyl...

CAS#:531-52-2

~%

2,3,5-Triphenyltetrazolium chloride Structure

2,3,5-Triphenyl...

CAS#:298-96-4

Literature: Journal of the American Chemical Society, , vol. 76, p. 1695
N-chlorosuccinimide structure

N-chlorosuccinimide

CAS#:128-09-6

Diazene, phenyl(phenyl(phenylhydrazono)methyl)- structure

Diazene, phenyl...

CAS#:531-52-2

~%

2,3,5-Triphenyltetrazolium chloride Structure

2,3,5-Triphenyl...

CAS#:298-96-4

Literature: Chemische Berichte, , vol. 86, p. 858,860
2-Chloroisoindoline-1,3-dione structure

2-Chloroisoindo...

CAS#:3481-09-2

Diazene, phenyl(phenyl(phenylhydrazono)methyl)- structure

Diazene, phenyl...

CAS#:531-52-2

~%

2,3,5-Triphenyltetrazolium chloride Structure

2,3,5-Triphenyl...

CAS#:298-96-4

Literature: Chemische Berichte, , vol. 86, p. 858,860
Hydrochloric acid structure

Hydrochloric acid

CAS#:7647-01-0

Diazene, phenyl(phenyl(phenylhydrazono)methyl)- structure

Diazene, phenyl...

CAS#:531-52-2

Isopentyl nitrite structure

Isopentyl nitrite

CAS#:110-46-3

~%

2,3,5-Triphenyltetrazolium chloride Structure

2,3,5-Triphenyl...

CAS#:298-96-4

Literature: Chemische Berichte, , vol. 27, p. 323,2928

 Customs

HS Code 2933990090
Summary 2933990090. heterocyclic compounds with nitrogen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

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 Synonyms

2,3,5-triphenyl tetrazolium chloride
TETRAZOLE RED
TTC
Red tetrazolium
triphenyltetrazolium chloride
urocheck
TPTZ,TTZ,Tetrazolium Red
TTL
EINECS 206-071-6
vitastain
2,3,5-Triphenyltetrazolium chloride
2,3,5-Triphenyl-2H-tetrazol-3-ium chloride
tetrazolium red
2H-Tetrazolium, 2,3,5-triphenyl-, chloride (1:1)
MFCD00011963
2,3,5-tetraphenyltetrazolium chloride
uroscreen
2,3,5-triphenyl-2H-tetrazolium chloride
TTZ
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