Janus Green B

Modify Date: 2024-01-04 09:59:36

Janus Green B Structure
Janus Green B structure
 Common Name Janus Green B
CAS Number 2869-83-2 Molecular Weight 511.060
Density N/A Boiling Point N/A
Molecular Formula C30H31ClN6 Melting Point 160 °C (dec.)(lit.)
MSDS USA Flash Point N/A

 Use of Janus Green B


Janus green B is a supravital stain. Janus green B staining reaction is oxygen dependent, and is reversibly inhibited by cyanide. Janus green B has been used for staining peripheral nerves in live insects, lymphatic vessels of rabbits and mitochondria[1][2][3].

 Names

Name Janus Green B chloride
Synonym More Synonyms

 Janus Green B Biological Activity

Description Janus green B is a supravital stain. Janus green B staining reaction is oxygen dependent, and is reversibly inhibited by cyanide. Janus green B has been used for staining peripheral nerves in live insects, lymphatic vessels of rabbits and mitochondria[1][2][3].
Related Catalog
References

[1]. LAZAROW A, et al. Studies on the enzymatic basis for the Janus green B staining reaction. J Histochem Cytochem. 1953 Jul;1(4):234-41.

[2]. Yack JE. Janus Green B as a rapid, vital stain for peripheral nerves and chordotonal organs in insects. J Neurosci Methods. 1993 Aug;49(1-2):17-22.

[3]. Lee BC, et al. Novel threadlike structures (Bonghan ducts) inside lymphatic vessels of rabbits visualized with a Janus Green B staining method. Anat Rec B New Anat. 2005 Sep;286(1):1-7.

 Chemical & Physical Properties

Melting Point 160 °C (dec.)(lit.)
Molecular Formula C30H31ClN6
Molecular Weight 511.060
Exact Mass 510.229858
PSA 47.97000
LogP 3.99630
Vapour Pressure 2.6 mm Hg ( 20 °C)
Water Solubility H2O: 1 M hot, clear, colorless

 Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :
SG1633000
CHEMICAL NAME :
Phenazinium, 3-(diethylamino)-7-((p-(dimethylamino)phenyl)azo)-5-p henyl-, chloride
CAS REGISTRY NUMBER :
2869-83-2
LAST UPDATED :
199701
DATA ITEMS CITED :
5
MOLECULAR FORMULA :
C30-H31-N6.Cl
MOLECULAR WEIGHT :
511.12
WISWESSER LINE NOTATION :
T C666 BK INJ BR& ENUNR DN1&1& MN2&2 &G

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Subcutaneous
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
960 mg/kg/13W-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria Tumorigenic - tumors at site of application

MUTATION DATA

TYPE OF TEST :
Cytogenetic analysis
TEST SYSTEM :
Rodent - hamster Ovary
DOSE/DURATION :
20 umol/L/5H (Continuous)
REFERENCE :
ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For publisher information, see EMMUEG. Volume(issue)/page/year: 1,27,1979 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - X1535 No. of Facilities: 181 (estimated) No. of Industries: 5 No. of Occupations: 5 No. of Employees: 2102 (estimated) No. of Female Employees: 188 (estimated)

 Safety Information

Personal Protective Equipment Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes T: Toxic;
Risk Phrases R60
Safety Phrases S22-S24/25
RIDADR NONH for all modes of transport
WGK Germany 3
RTECS SG1633000
HS Code 32041900

 Customs

HS Code 32041900

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 Synonyms

3-(Diethylamino)-7-{(E)-[4-(dimethylamino)phenyl]diazenyl}-5-phenylphenazin-5-ium chloride
Phenazinium, 3-(diethylamino)-7-[(E)-2-[4-(dimethylamino)phenyl]diazenyl]-5-phenyl-, chloride (1:1)
Janus Green V
Janus Green B,high purity biological stain
8-[[4-(dimethylamino)phenyl]diazenyl]-N,N-diethyl-10-phenylphenazin-10-ium-2-amine,chloride
Janus Green B
EINECS 220-695-6
MFCD00011337
3-(Diethylamino)-7-[[4-(dimethylamino)phenyl]azo]-5-phenylphenazinium Chloride
diazin green s
Phenazinium, 3- (diethylamino)-7-[[p-(dimethylamino)phenyl]azo]-5-phenyl-, chloride
Phenazinium, 3- (diethylamino)-7-[[4-(dimethylamino)phenyl]azo]-5-phenyl-, chloride
Union Green B
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