fluorescein 5-isothiocyanate

Modify Date: 2025-08-26 16:44:41

fluorescein 5-isothiocyanate Structure
fluorescein 5-isothiocyanate structure
 Common Name fluorescein 5-isothiocyanate
CAS Number 27072-45-3 Molecular Weight 389.381
Density 1.5±0.1 g/cm3 Boiling Point 708.6±60.0 °C at 760 mmHg
Molecular Formula C21H11NO5S Melting Point >360ºC(lit.)
MSDS Chinese USA Flash Point 382.4±32.9 °C
Symbol GHS08
GHS08		 Signal Word Danger

 Use of fluorescein 5-isothiocyanate


5(6)-FITC (Fluorescein isothiocyanate) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA.

 Names

Name Fluorescein isothiocyanate
Synonym More Synonyms

 fluorescein 5-isothiocyanate Biological Activity

Description 5(6)-FITC (Fluorescein isothiocyanate) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA.
Related Catalog
References

[1]. Goding JW. Conjugation of antibodies with fluorochromes: modifications to the standard methods. J Immunol Methods. 1976;13(3-4):215-26.

[2]. Der-Balian GP, et al. Fluorescein labeling of Fab' while preserving single thiol. Anal Biochem. 1988 Aug 15;173(1):59-63.

[3]. Smith LM, et al. Fluorescence detection in automated DNA sequence analysis. Nature. 1986 Jun 12-18;321(6071):674-9.

 Chemical & Physical Properties

Density 1.5±0.1 g/cm3
Boiling Point 708.6±60.0 °C at 760 mmHg
Melting Point >360ºC(lit.)
Molecular Formula C21H11NO5S
Molecular Weight 389.381
Flash Point 382.4±32.9 °C
Exact Mass 389.035797
PSA 120.44000
LogP 4.00
Appearance of Characters powder
Vapour Pressure 0.0±2.3 mmHg at 25°C
Index of Refraction 1.754
Storage condition 2-8°C

 Safety Information

Symbol GHS08
GHS08
Signal Word Danger
Hazard Statements H334
Precautionary Statements P261-P342 + P311
Personal Protective Equipment dust mask type N95 (US);Eyeshields;Faceshields;Gloves
Hazard Codes Xn: Harmful;
Risk Phrases R42/43
Safety Phrases S22
RIDADR NONH for all modes of transport
WGK Germany 3

 Articles2410

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 Synonyms

5-FITC
Isothiocyanatofluorescein
5-Isothionatofluorescein
FITC isomer I
Fluorescein Isothiosyanate
fluorescein isothiocyanate isomer 1
FITC ISOMER
EINECS 222-042-0
3',6'-Dihydroxy-5-isothiocyanato-3H-spiro[2-benzofurane-1,9'-xanthén]-3-one
Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 3',6'-dihydroxy-5-isothiocyanato-
FITC-I
Fluorescein 5(6)-isothiocyanate
FITC
Fluorescein-5-isothiocyanate
MFCD00005063
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5-isothiocyanato-
Fluorescein Isothiocyanate
fluorescein 5-isothiocyanate
3',6'-Dihydroxy-5-isothiocyanato-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one
5(6)-FITC
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