X-glucuronide CHA salt

Modify Date: 2024-01-11 16:58:37

X-glucuronide CHA salt Structure
X-glucuronide CHA salt structure
Common Name X-glucuronide CHA salt
CAS Number 114162-64-0 Molecular Weight 521.787
Density 1.4241 (rough estimate) Boiling Point 732.2ºC at 760 mmHg
Molecular Formula C20H26BrClN2O7 Melting Point 228-230ºC
MSDS USA Flash Point 396.6ºC

 Use of X-glucuronide CHA salt


X-Gluc cyclohexanamine is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc cyclohexanamine can be used to detect E. coli contamination in food, water and the urinary tract (GUS as a specific detection indicator). X-Gluc cyclohexanamine is also widely used in molecular biology experiments to label and detect the expression of target genes (reacts with the GUS gene, appears blue)[1].

 Names

Name 5-bromo-4-chloro-3-indolyl b-d
Synonym More Synonyms

 X-glucuronide CHA salt Biological Activity

Description X-Gluc cyclohexanamine is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc cyclohexanamine can be used to detect E. coli contamination in food, water and the urinary tract (GUS as a specific detection indicator). X-Gluc cyclohexanamine is also widely used in molecular biology experiments to label and detect the expression of target genes (reacts with the GUS gene, appears blue)[1].
Related Catalog
In Vitro Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Dissolve 20 mg X-Gluc cyclohexanamine in 1mL dimethylformamide (DMF) to prepare X-Gluc master mix. 2. Add the prepared X-Gluc solution to agar medium plates at a final concentration of 50 μg/mL without sterilization. 3. Allow the plates to air dry and be used to inoculate the organisms. 4. Incubate the plates at 35°C and observe 16-24 h after inoculation.
References

[1]. Elon W Frampton, et al. Evaluation of the β-Glucuronidase Substrate 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide (X-GLUC) in a 24-Hour Direct Plating Method for Escherichia coli. J Food Prot. 1988 May;51(5):402-404.  

 Chemical & Physical Properties

Density 1.4241 (rough estimate)
Boiling Point 732.2ºC at 760 mmHg
Melting Point 228-230ºC
Molecular Formula C20H26BrClN2O7
Molecular Weight 521.787
Flash Point 396.6ºC
Exact Mass 520.061157
PSA 158.26000
LogP 2.83290
Vapour Pressure 1.66E-22mmHg at 25°C
Index of Refraction 1.6110 (estimate)
Storage condition 2-8°C
Water Solubility DMF: soluble

 Safety Information

Personal Protective Equipment Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes Xi: Irritant;
Risk Phrases R36/37/38
Safety Phrases S26-S37/39
RIDADR NONH for all modes of transport
WGK Germany 3
HS Code 29349990

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 Synonyms

X-Gluc CHA Salt
5-Bromo-4-chloro-3-indolyl-β-D-glucuronic acid cyclohexylammonium salt
5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt
5-Bromo-4-chloro-3-indolyl Beta-D-glucuronide cyclohexylammonium salt
5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt hydrate
Cyclohexanaminium 5-bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronate
X-glucuronide CHA salt
X-gluc/X-GlcA
X-Glu,CHA Salt
5-Bromo-4-chlo
X-GlcA CHA Salt
β-D-Glucopyranosiduronic acid, 5-bromo-4-chloro-1H-indol-3-yl, compd. with cyclohexanamine (1:1)
Cyclohexanamine - 5-bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronic acid (1:1)
5-Bromo-4-chloro-3-indolyl-β-D-glucuronide cyclohexylammonium salt
X-GlcA,X-glucuronide CHA salt
5-BROMO-4-CHLORO-3-INDOLYLB-D-GLUCURONID E
5-Bromo-4-chloro-3-indolyl β-D-Glucopyranosiduronic Acid Cyclohexylammonium Salt
5-BROMO-4-CHLORO-3-INDOLYL-B-D-GLUCURO-N IDE CYCLOHEXYLA.S.