X-glucuronide CHA salt

Names

[ Name ]:
X-glucuronide CHA salt

[Synonym ]:
X-Gluc CHA Salt
5-Bromo-4-chloro-3-indolyl-β-D-glucuronic acid cyclohexylammonium salt
5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt
5-Bromo-4-chloro-3-indolyl Beta-D-glucuronide cyclohexylammonium salt
5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt hydrate
Cyclohexanaminium 5-bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronate
X-glucuronide CHA salt
X-gluc/X-GlcA
X-Glu,CHA Salt
5-Bromo-4-chlo
X-GlcA CHA Salt
β-D-Glucopyranosiduronic acid, 5-bromo-4-chloro-1H-indol-3-yl, compd. with cyclohexanamine (1:1)
Cyclohexanamine - 5-bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronic acid (1:1)
5-Bromo-4-chloro-3-indolyl-β-D-glucuronide cyclohexylammonium salt
X-GlcA,X-glucuronide CHA salt
5-BROMO-4-CHLORO-3-INDOLYLB-D-GLUCURONID E
5-Bromo-4-chloro-3-indolyl β-D-Glucopyranosiduronic Acid Cyclohexylammonium Salt
5-BROMO-4-CHLORO-3-INDOLYL-B-D-GLUCURO-N IDE CYCLOHEXYLA.S.

Biological Activity

[Description]:

X-Gluc cyclohexanamine is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc cyclohexanamine can be used to detect E. coli contamination in food, water and the urinary tract (GUS as a specific detection indicator). X-Gluc cyclohexanamine is also widely used in molecular biology experiments to label and detect the expression of target genes (reacts with the GUS gene, appears blue)[1].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[In Vitro]

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Dissolve 20 mg X-Gluc cyclohexanamine in 1mL dimethylformamide (DMF) to prepare X-Gluc master mix. 2. Add the prepared X-Gluc solution to agar medium plates at a final concentration of 50 μg/mL without sterilization. 3. Allow the plates to air dry and be used to inoculate the organisms. 4. Incubate the plates at 35°C and observe 16-24 h after inoculation.

[References]

[1]. Elon W Frampton, et al. Evaluation of the β-Glucuronidase Substrate 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide (X-GLUC) in a 24-Hour Direct Plating Method for Escherichia coli. J Food Prot. 1988 May;51(5):402-404.

Chemical & Physical Properties

[ Density]:
1.4241 (rough estimate)

[ Boiling Point ]:
732.2ºC at 760 mmHg

[ Melting Point ]:
228-230ºC

[ Molecular Formula ]:
C₂₀H₂₆BrClN₂O₇

[ Molecular Weight ]:
521.787

[ Flash Point ]:
396.6ºC

[ Exact Mass ]:
520.061157

[ PSA ]:
158.26000

[ LogP ]:
2.83290

[ Vapour Pressure ]:
1.66E-22mmHg at 25°C

[ Index of Refraction ]:
1.6110 (estimate)

[ Storage condition ]:
2-8°C

[ Water Solubility ]:
DMF: soluble

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Hazard Codes ]:
Xi: Irritant;

[ Risk Phrases ]:
R36/37/38

[ Safety Phrases ]:
S26-S37/39

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

[ HS Code ]:
29349990

Articles

Automated Sampling Procedures Supported by High Persistence of Bacterial Fecal Indicators and Bacteroidetes Genetic Microbial Source Tracking Markers in Municipal Wastewater during Short-Term Storage at 5°C.

Appl. Environ. Microbiol. 81 , 5134-43, (2015)

Because of high diurnal water quality fluctuations in raw municipal wastewater, the use of proportional autosampling over a period of 24 h at municipal wastewater treatment plants (WWTPs) to evaluate ...

Quality changes of fresh-cut pomegranate arils during shelf life as affected by deficit irrigation and postharvest vapour treatments.

J. Sci. Food Agric. 95 , 2325-36, (2015)

The effect of two sustained deficit irrigation (SDI) strategies, compared to a control, on postharvest physicochemical, microbial, sensory quality attributes and anthocyanin content of fresh-cut pomeg...

Comparison of Six Chromogenic Agar Media for the Isolation of a Broad Variety of Non-O157 Shigatoxin-Producing Escherichia coli (STEC) Serogroups.

Int. J. Environ. Res. Public Health 12 , 6965-78, (2015)

The isolation of non-O157 STEC from food samples has proved to be challenging. The selection of a suitable selective isolation agar remains problematic. The purpose of this study was to qualitatively ...