5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt

Modify Date: 2024-01-03 17:04:16

5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt Structure
5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt structure
Common Name 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt
CAS Number 129541-41-9 Molecular Weight 521.787
Density N/A Boiling Point 732.2ºC at 760mmHg
Molecular Formula C20H26BrClN2O7 Melting Point 228-230°C
MSDS Chinese USA Flash Point 396.6ºC

 Use of 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt


X-Gluc sodium is a dye reagent for the detection of β-glucuronidase, an enzyme produced by Escherichia coli. X-Gluc sodium can be used to detect E. coli contamination in food, water and the urinary tract. X-Gluc sodium is also widely used in molecular biology experiments to label and detect the expression of target genes (GUS reporter system)[1].

 Names

Name 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt
Synonym More Synonyms

  Biological Activity

Description X-Gluc sodium is a dye reagent for the detection of β-glucuronidase, an enzyme produced by Escherichia coli. X-Gluc sodium can be used to detect E. coli contamination in food, water and the urinary tract. X-Gluc sodium is also widely used in molecular biology experiments to label and detect the expression of target genes (GUS reporter system)[1].
Related Catalog
In Vitro Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Dissolve 20 mg X-Gluc sodium in 1mL dimethylformamide (DMF) to prepare X-Gluc master mix. 2. Add the prepared X-Gluc solution to agar medium plates at a final concentration of 50 μg/mL without sterilization. 3. Allow the plates to air dry and be used to inoculate the organisms. 4. Incubate the plates at 35°C and observe 16-24 h after inoculation.
References

[1]. Elon W Frampton, et al. Evaluation of the β-Glucuronidase Substrate 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide (X-GLUC) in a 24-Hour Direct Plating Method for Escherichia coli. J Food Prot. 1988 May;51(5):402-404.

 Chemical & Physical Properties

Boiling Point 732.2ºC at 760mmHg
Melting Point 228-230°C
Molecular Formula C20H26BrClN2O7
Molecular Weight 521.787
Flash Point 396.6ºC
Exact Mass 520.061157
PSA 135.07000
Storage condition −20°C

 Safety Information

Personal Protective Equipment Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes Xi
Risk Phrases R36/37/38
Safety Phrases S24/25
RIDADR NONH for all modes of transport
WGK Germany 3
HS Code 29339990

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 Synonyms

5-Bromo-4-chloro-3-indolyl β-D-Glucopyranosiduronic Acid Sodium Salt
5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt
5-Bromo-4-chloro-3-indolyl Beta-D-glucuronide cyclohexylammonium salt
5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt hydrate
BC-Indicator,X-GlcA,X-glucuronide
X-gluc/X-GlcA
X-Glu,CHA Salt
X-Gluc Sodium Salt
Cyclohexanamine - 5-bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronic acid (1:1)
X-GlcA,X-glucuronide CHA salt
5-Bromo-4-chloro-3-indolyl β-D-Glucuronide Sodium Salt
5-BROMO-4-CHLORO-3-INDOLYLB-D-GLUCURONID E
5-Bromo-4-chloro-3-indolyl β-D-Glucopyranosiduronic Acid Cyclohexylammonium Salt
5-BROMO-4-CHLORO-3-INDOLYL-B-D-GLUCURO-N IDE CYCLOHEXYLA.S.
X-Gluc CHA Salt
X-GlcA Sodium Salt
5-Bromo-4-chloro-3-indolyl-β-D-glucuronic acid cyclohexylammonium salt
5-Bromo-4-chloro-3-indolyl-β-D-glucuronide sodium salt
5-BroMo-4-chloro-3-indolyl beta-D-Glucuronide SodiuM Salt
Cyclohexanaminium 5-bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronate
X-glucuronide CHA salt
5-Bromo-4-chloro-3-indolyl-b-D-glucuronide sodium salt
5-Bromo-4-chlo
X-GlcA CHA Salt
β-D-Glucopyranosiduronic acid, 5-bromo-4-chloro-1H-indol-3-yl, compd. with cyclohexanamine (1:1)
5-Bromo-4-chloro-3-indolyl-β-D-glucuronide cyclohexylammonium salt
MFCD00135782
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