Basic Orange 14

Modify Date: 2024-01-02 15:26:47

Basic Orange 14 Structure
Basic Orange 14 structure
Common Name Basic Orange 14
CAS Number 10127-02-3 Molecular Weight 739.943
Density N/A Boiling Point 468.6ºC at 760 mmHg
Molecular Formula C34H40Cl4N6Zn Melting Point 165 °C
MSDS Chinese USA Flash Point 237.2ºC
Symbol GHS08
GHS08
Signal Word Warning

 Use of Basic Orange 14


Acridine Orange (Euchrysine 3RX) zinc chloride salt is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange zinc chloride salt produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm)[1][2][3].

 Names

Name Basic Orange 14
Synonym More Synonyms

 Basic Orange 14 Biological Activity

Description Acridine Orange (Euchrysine 3RX) zinc chloride salt is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange zinc chloride salt produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm)[1][2][3].
Related Catalog
In Vitro Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[4]. 1. 用 Acridine Orange zinc chloride salt 染色细胞 (1 μM; 20 分钟; 37 ℃)。 2. 用 PBS 洗涤细胞。 3. 通过共聚焦激光扫描显微镜 (FV3000,Olympus) 观察细胞。
In Vivo Acridine Orange (0.1 mg/kg,静脉注射,狗) zinc chloride salt 在 30 天内没有表现出临床毒性迹象,血液中也没有发现异常[1]。
References

[1]. McMaster GK, et al. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarosegels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8.  

[2]. Traganos F, et al. Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. J Histochem Cytochem. 1977 Jan;25(1):46-56.  

[3]. Byvaltsev VA, et al. Acridine Orange: A Review of Novel Applications for Surgical Cancer Imaging and Therapy. Front Oncol. 2019 Sep 24;9:925.  

[4]. Wang Q, et al. Substrate stiffness regulates the differentiation profile and functions of osteoclasts via cytoskeletal arrangement. Cell Prolif. 2022 Jan;55(1):e13172.  

 Chemical & Physical Properties

Boiling Point 468.6ºC at 760 mmHg
Melting Point 165 °C
Molecular Formula C34H40Cl4N6Zn
Molecular Weight 739.943
Flash Point 237.2ºC
Exact Mass 736.135986
PSA 38.74000
LogP 10.02050
Storage condition Store at RT.
Water Solubility soluble

 Safety Information

Symbol GHS08
GHS08
Signal Word Warning
Hazard Statements H341
Precautionary Statements P281
Personal Protective Equipment Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes Xn:Harmful;
Risk Phrases R20/21/22;R68
Safety Phrases S45-S36/37
RIDADR NONH for all modes of transport
WGK Germany 3
RTECS AR7600000
Packaging Group I; II; III
Hazard Class 6.1
HS Code 32041300

 Customs

HS Code 32041300

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 Synonyms

N,N,N',N'-Tetramethyl-3,6-acridinediamine - dichlorozinc (2:1) dihydrochloride
Acridine Orange hemi(zinc chloride) salt
MFCD00081043
zinc,3-N,3-N,6-N,6-N-tetramethylacridine-3,6-diamine,dichloride,hydrochloride
Acridine Orange
N,N,N',N'-Tetramethyl-3,6-acridinediamine - dichlorozinc hydrochloride (2:1:2)
Euchrysin GNY
EINECS 200-614-0
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