Basic Orange 14

Names

[ Name ]:
Basic Orange 14

[Synonym ]:
N,N,N',N'-Tetramethyl-3,6-acridinediamine - dichlorozinc (2:1) dihydrochloride
Acridine Orange hemi(zinc chloride) salt
MFCD00081043
zinc,3-N,3-N,6-N,6-N-tetramethylacridine-3,6-diamine,dichloride,hydrochloride
Acridine Orange
N,N,N',N'-Tetramethyl-3,6-acridinediamine - dichlorozinc hydrochloride (2:1:2)
Euchrysin GNY
EINECS 200-614-0

Biological Activity

[Description]:

Acridine Orange (Euchrysine 3RX) zinc chloride salt is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange zinc chloride salt produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm)[1][2][3].

[Related Catalog]:

Research Areas >> Others

[In Vitro]

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[4]. 1. 用 Acridine Orange zinc chloride salt 染色细胞 (1 μM; 20 分钟; 37 ℃)。 2. 用 PBS 洗涤细胞。 3. 通过共聚焦激光扫描显微镜 (FV3000，Olympus) 观察细胞。

[In Vivo]

Acridine Orange (0.1 mg/kg，静脉注射，狗) zinc chloride salt 在 30 天内没有表现出临床毒性迹象，血液中也没有发现异常[1]。

[References]

[1]. McMaster GK, et al. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarosegels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8.

[2]. Traganos F, et al. Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. J Histochem Cytochem. 1977 Jan;25(1):46-56.

[3]. Byvaltsev VA, et al. Acridine Orange: A Review of Novel Applications for Surgical Cancer Imaging and Therapy. Front Oncol. 2019 Sep 24;9:925.

[4]. Wang Q, et al. Substrate stiffness regulates the differentiation profile and functions of osteoclasts via cytoskeletal arrangement. Cell Prolif. 2022 Jan;55(1):e13172.

Chemical & Physical Properties

[ Boiling Point ]:
468.6ºC at 760 mmHg

[ Melting Point ]:
165 °C

[ Molecular Formula ]:
C₃₄H₄₀Cl₄N₆Zn

[ Molecular Weight ]:
739.943

[ Flash Point ]:
237.2ºC

[ Exact Mass ]:
736.135986

[ PSA ]:
38.74000

[ LogP ]:
10.02050

[ Storage condition ]:
Store at RT.

[ Water Solubility ]:
soluble

MSDS

Click to get detail MSDS

Safety Information

[ Symbol ]:

GHS08

[ Signal Word ]:
Warning

[ Hazard Statements ]:
H341

[ Precautionary Statements ]:
P281

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Hazard Codes ]:
Xn:Harmful;

[ Risk Phrases ]:
R20/21/22;R68

[ Safety Phrases ]:
S45-S36/37

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

[ RTECS ]:
AR7600000

[ Packaging Group ]:
I; II; III

[ Hazard Class ]:
6.1

[ HS Code ]:
32041300

Customs

[ HS Code ]: 32041300

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