10127-02-3

10127-02-3 structure

Name: Basic Orange 14
Chemical Name: Basic Orange 14
CAS Number: 10127-02-3
Molecular Formula: C₃₄H₄₀Cl₄N₆Zn
Molecular Weight: 739.943
Catalog: Dyes and pigments dye Basic dye
Create Date: 2018-09-02 18:19:26
Modify Date: 2024-01-02 15:26:47
Acridine Orange (Euchrysine 3RX) zinc chloride salt is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange zinc chloride salt produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm)[1][2][3].

Name	Basic Orange 14
Synonyms	N,N,N',N'-Tetramethyl-3,6-acridinediamine - dichlorozinc (2:1) dihydrochloride Acridine Orange hemi(zinc chloride) salt MFCD00081043 zinc,3-N,3-N,6-N,6-N-tetramethylacridine-3,6-diamine,dichloride,hydrochloride Acridine Orange N,N,N',N'-Tetramethyl-3,6-acridinediamine - dichlorozinc hydrochloride (2:1:2) Euchrysin GNY EINECS 200-614-0

Description	Acridine Orange (Euchrysine 3RX) zinc chloride salt is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange zinc chloride salt produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm)[1][2][3].
Related Catalog	Research Areas >> Others
In Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[4]. 1. 用 Acridine Orange zinc chloride salt 染色细胞 (1 μM; 20 分钟; 37 ℃)。 2. 用 PBS 洗涤细胞。 3. 通过共聚焦激光扫描显微镜 (FV3000，Olympus) 观察细胞。
In Vivo	Acridine Orange (0.1 mg/kg，静脉注射，狗) zinc chloride salt 在 30 天内没有表现出临床毒性迹象，血液中也没有发现异常[1]。
References	[1]. McMaster GK, et al. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarosegels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8. [2]. Traganos F, et al. Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. J Histochem Cytochem. 1977 Jan;25(1):46-56. [3]. Byvaltsev VA, et al. Acridine Orange: A Review of Novel Applications for Surgical Cancer Imaging and Therapy. Front Oncol. 2019 Sep 24;9:925. [4]. Wang Q, et al. Substrate stiffness regulates the differentiation profile and functions of osteoclasts via cytoskeletal arrangement. Cell Prolif. 2022 Jan;55(1):e13172.

Boiling Point	468.6ºC at 760 mmHg
Melting Point	165 °C
Molecular Formula	C₃₄H₄₀Cl₄N₆Zn
Molecular Weight	739.943
Flash Point	237.2ºC
Exact Mass	736.135986
PSA	38.74000
LogP	10.02050
Storage condition	Store at RT.
Water Solubility	soluble

Symbol	GHS08
Signal Word	Warning
Hazard Statements	H341
Precautionary Statements	P281
Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes	Xn:Harmful;
Risk Phrases	R20/21/22;R68
Safety Phrases	S45-S36/37
RIDADR	NONH for all modes of transport
WGK Germany	3
RTECS	AR7600000
Packaging Group	I; II; III
Hazard Class	6.1
HS Code	32041300

HS Code	32041300

10127-02-3	11075-16-4
61901-61-9	39279-58-8
3056-93-7	37291-40-0
132758-56-6	12217-46-8
532-82-1	6368-70-3