1226759-47-2

1226759-47-2 structure

Name: LCL521 dihydrochloride
Chemical Name: LCL521 dihydrochloride
CAS Number: 1226759-47-2
Molecular Formula: C₃₁H₅₄Cl₂N₄O₇
Molecular Weight: 665.69
Catalog: Research Areas Cancer
Create Date: 2019-02-08 18:36:43
Modify Date: 2025-08-25 16:35:23
LCL521 dihydrochloride is an acid ceramidase (ACDase) inhibitor. LCL521 also inhibits the lysosomal acid sphingomyelinase (ASMase).

Name	LCL521 dihydrochloride

Description	LCL521 dihydrochloride is an acid ceramidase (ACDase) inhibitor. LCL521 also inhibits the lysosomal acid sphingomyelinase (ASMase).
Related Catalog	Signaling Pathways >> Others >> Others Research Areas >> Cancer
Target	ACDase, ASMase[1]
In Vitro	Acid ceramidase (ACDase) is being recognized as a therapeutic target for cancer. B13 represents a moderate inhibitor of ACDase. LCL521 applied at a lower concentration, 1 µM, shows an effect similar to 10µM treatment, suggesting a high efficiency on this enzyme inhibition. LCL521 causes a time-dependent decrease of the α-ACDase form. 1 µM LCL521 acts as a potent inhibitor of cellular ACDase activity, whereas 10 µM LCL521 has an additional, decreased affect on the α-form of this enzyme. Moreover, LCL521 acts as inhibitor of the lysosomal ACDase and ASMase[1].
Cell Assay	HeLa cells overexpressing ASAH2, ACER2, or empty vector are grown to a 60% confluence before they are treated with Tetracycline (5 ng/mL) overnight. Next day, the cells are then treated with B13 (10 µM), LCL521 (1 or 10 µM), or Ethanol (the vehicle control for both B13 and LCL521) for 1 h before they are harvested[1].
References	[1]. Bai A, et al. Targeting (cellular) lysosomal acid ceramidase by B13: design, synthesis and evaluation of novel DMG-B13 ester prodrugs. Bioorg Med Chem. 2014 Dec 15;22(24):6933-44.