1429617-90-2
1429617-90-2 structure
- Name: DDR1 Inhibitor 7rh
- Chemical Name: DDR1-IN-2
- CAS Number: 1429617-90-2
- Molecular Formula: C30H29F3N6O
- Molecular Weight: 546.586
- Catalog: Signaling Pathways Protein Tyrosine Kinase/RTK Discoidin Domain Receptor
- Create Date: 2018-01-31 01:23:43
- Modify Date: 2024-01-14 11:04:21
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DDR1-IN-2 is a potent inhibitor of discoidin domain receptor 1 (DDR1), with an IC50 of 13.1 nM, and also less potently inhibits DDR2, with an IC50 of 203 nM.
| Name | DDR1-IN-2 |
|---|---|
| Synonyms |
4-Ethyl-N-{3-[(4-methyl-1-piperazinyl)methyl]-5-(trifluoromethyl)phenyl}-3-(pyrazolo[1,5-a]pyrimidin-6-ylethynyl)benzamide
Benzamide, 4-ethyl-N-[3-[(4-methyl-1-piperazinyl)methyl]-5-(trifluoromethyl)phenyl]-3-(2-pyrazolo[1,5-a]pyrimidin-6-ylethynyl)- |
| Description | DDR1-IN-2 is a potent inhibitor of discoidin domain receptor 1 (DDR1), with an IC50 of 13.1 nM, and also less potently inhibits DDR2, with an IC50 of 203 nM. |
|---|---|
| Related Catalog | |
| Target |
IC50: 13.1 nM (DDR1), 203 nM (DDR2), 414 nM (Bcr-Abl), 2500 nM (c-Kit)[1] |
| In Vitro | DDR1-IN-2 (compound 1) is a potent inhibitor of DDR1, with an IC50 of 13.1 nM, and less potently inhibits DDR2, with an IC50 of 203 nM. DDR1-IN-2 also shows inhibitory activities against Bcr-Abl and c-Kit, with IC50s of 414 and 2500 nM, respectively[1]. |
| Kinase Assay | The effects of compounds (including DDR1-IN-2) on the kinases DDR1 and DDR2 are assessed by using a LanthaScreen Eu kinase activity assay technology. Kinase reactions are performed in a 10 µL solution in low-volume 384-well plates. The kinase reaction buffer consists of 50 mM HEPES pH 7.5, 0.01% BRIJ-35, 10 mM MgCl2, and 1 mM EGTA; the concentration of Fluorescein-Poly GAT substrate in the assay is 100 nM. Kinase reactions are initiated by the addition of 100 nM ATP in the presence of serially diluted compounds (DDR1-IN-2). The reactions are allowed to proceed for 1 h at room temperature before a 10 µL preparation of EDTA (20 mM) and Eu-labeled antibody (4 nM) in TR-FRET dilution buffer are added. The final concentration of antibody in the assay well is 2 nM, and the final concentration of EDTA is 10 mM. The plate is allowed to incubate at room temperature for one more hour before the TR-FRET emission ratios of 665 nm/340 nm are acquired on a multilabel reader. Data analysis and curve fitting are performed using GraphPad Prism4 software[1]. |
| References |
| Density | 1.3±0.1 g/cm3 |
|---|---|
| Molecular Formula | C30H29F3N6O |
| Molecular Weight | 546.586 |
| Exact Mass | 546.235474 |
| LogP | 4.32 |
| Index of Refraction | 1.616 |
| Storage condition | 2-8℃ |