35340-49-9
35340-49-9 structure
- Name: Dihydrofluorescein diacetate
- Chemical Name: 2-(3,6-diacetyloxy-9H-xanthen-9-yl)benzoic acid
- CAS Number: 35340-49-9
- Molecular Formula: C24H18O7
- Molecular Weight: 418.39600
- Catalog: Biochemical Biochemical reagent Biological dye
- Create Date: 2018-03-11 08:00:00
- Modify Date: 2024-01-10 12:01:03
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Dihydrofluorescein diacetate is a fluorimetric probe mainly used for oxidative stress measurements, in both cell-free systems and cellular models.
| Name | 2-(3,6-diacetyloxy-9H-xanthen-9-yl)benzoic acid |
|---|---|
| Synonyms |
MFCD00005056
Dihydrofluorescein diacetate 2-[3,6-Diacetoxy-9H-xanth-9-yl]benzoic acid 2-[3,6-Bis(acetyloxy)-9H-xanthen-9-yl]benzoic acid D1845 2-(3,6-diacetoxy-xanthen-9-yl)-benzoic acid 2-(3,6-Diacetoxy-xanthen-9-yl)-benzoesaeure Diacetyldihydrofluorescein |
| Description | Dihydrofluorescein diacetate is a fluorimetric probe mainly used for oxidative stress measurements, in both cell-free systems and cellular models. |
|---|---|
| Related Catalog | |
| In Vitro | Dihydrofluorescein diacetate may be a superior fluorescent probe for many cell-based studies. It is a better fluorescent probe for detecting intracellular oxidants because it is more reactive toward specific oxidizing species. Dihydrofluorescein diacetate demonstrates fluorescence of linear structures, consistent with mitochondria, in reoxygenated endothelium[1]. Dihydrofluorescein diacetate is able to detect the presence of ROS in mitochondria. Dihydrofluorescein diacetate fluorescence was sharp and delineated thin filaments which corresponded in all details to TMRM-stained mitochondria. It enters mitochondria and reacts with ROS released in the matrix[2]. Dihydrofluorescein diacetate could be an useful and quantitative method for measuring the oxidative potential of nanoparticle-treated cells[3]. |
| Cell Assay | Dihydrofluorescein diacetate is used at a final concentration of 5 μM for the cell-free studies. The excitation wavelength is 488 nm and the emission wavelength is 521 nm, the same as the excitation and emission wavelengths of the confocal microscope. All fluorescence measurements are performed on room temperature solutions. Human umbilical vein endothelium at first passage are cultured to confluence on 48 well plates. Cells are ished with PBS, then PBS with glucose, pH 7.4, is added to the cells. Dihydrofluorescein diacetate is added at a final concentration of 20 mM. Wells without cells are also loaded with fluorescent probes to serve as controls. Fluorescence measurements are made on a fluorescent platereader every 10 min at Exλ 488 nm and Emλ 510 (bandpass 5 nm) and 538 nm (bandpass 10 nm). The Emλ 510 nm and Emλ 538 nm values are added together[1]. |
| References |
| Density | 1.353g/cm3 |
|---|---|
| Boiling Point | 554.3ºC at 760 mmHg |
| Melting Point | 213-215ºC(lit.) |
| Molecular Formula | C24H18O7 |
| Molecular Weight | 418.39600 |
| Exact Mass | 418.10500 |
| PSA | 99.13000 |
| LogP | 4.52130 |
| Index of Refraction | 1.626 |
| Storage condition | 2-8℃ |
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
|---|---|
| Safety Phrases | S22-S24/25 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
| HS Code | 2932999099 |
|
~94%
35340-49-9 |
| Literature: Sharma, Padam N.; Brossi, Arnold Helvetica Chimica Acta, 1984 , vol. <1> 67, # 35 p. 301 - 304 |
|
~%
35340-49-9 |
| Literature: Heterocycles, , vol. 31, # 11 p. 1975 - 1982 |
|
~%
35340-49-9 |
| Literature: Heterocycles, , vol. 31, # 11 p. 1975 - 1982 |
|
~%
35340-49-9 |
| Literature: Monatshefte fuer Chemie, , vol. 13, p. 417 |
| HS Code | 2932999099 |
|---|---|
| Summary | 2932999099. other heterocyclic compounds with oxygen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0% |