(Gly14)-Humanin (human) trifluoroacetate salt

(Gly14)-Humanin (human) (14-Glycine-Humanin (human)) is an analog of Humanin in which the 14th amino acid serine was replaced with glycine (Gly). (Gly14)-Humanin (human) has anti-apoptotic and neuroprotective functions[1][2].

Signaling Pathways >> Apoptosis >> Apoptosis

Research Areas >> Neurological Disease

(Gly14)-Humanin (human) (0.1-10 μM; 72 hours) 显着增加细胞活力，降低 HUVEC 的核荧光、裂解 PARP 水平、ROS 形成和 bax/bcl-2 比值 。并降低 bax 的mRNA水平，增加 bcl-2 的 mRNA 水平[1]。 Cell Viability Assay[1] Cell Line: Human umbilical vein endothelial cells (HUVECs) Concentration: 100 nM, 1 μM and 10 μM Incubation Time: Pretreatment 3 hours and then treated with 72 hours Result: Significantly increased cell viability. Western Blot Analysis[1] Cell Line: Human umbilical vein endothelial cells (HUVECs) Concentration: 1 μM Incubation Time: Pretreatment 3 hours and then treated with 72 hours Result: Reduced the ratio of bax/bcl-2.

(Gly14)-Humanin (human)（0.1 μg/5 μL；i.c.v.；一次）减少受损皮层和海马体中具有质膜通透性的细胞，减少脑损伤体积，改善运动性能并改善莫里斯水迷宫试验中的性能[2]。 Animal Model: Adult male CD-1 mice (25-30 g; 10-12-week-old) bearing traumatic brain injury (TBI)[2] Dosage: 0.1 μg/5 μL Administration: Intracerebroventricularly (i.c.v.); once Result: Decreased cells with plasmalemma permeability in the injured cortex and hippocampus, reduced brain lesion volume, improved motor performance.

[1]. Ying Xie, et al. Protection effect of [Gly14]-Humanin from apoptosis induced by high glucose in human umbilical vein endothelial cells. Diabetes Res Clin Pract. 2014 Dec;106(3):560-6.  

[2]. T Wang, et al. [Gly14]-Humanin reduces histopathology and improves functional outcome after traumatic brain injury in mice. Neuroscience. 2013 Feb 12;231:70-81.