Calmodulin-Dependent Protein Kinase II (290-309)

Names

[ Name ]:
Calmodulin-Dependent Protein Kinase II (290-309)

[Synonym ]:
CAM KINASE II (290-309)
CALMODULIN-DEPENDENT PROTEIN KINASE II (290-309)
CaM kinase II (290-309)
CALMODULIN-DEPENDENT PROTEIN KINASE II FRAGMENT 290-309
LKKFNARRKLKGAILTTMLA
CALMODULIN DEPENDENT PROTEIN KINASE II
CALMODULIN BINDING DOMAIN
LEU-LYS-LYS-PHE-ASN-ALA-ARG-ARG-LYS-LEU-LYS-GLY-ALA-ILE-LEU-THR-THR-MET-LEU-ALA
CA2+/CALMODULIN-DEPENDENT PROTEIN KINASE II (290-309)
CALMODULIN-DEPENDENT PROTEIN KINASE IIFR AGMENT 290
H-LEU-LYS-LYS-PHE-ASN-ALA-ARG-ARG-LYS-LEU-LYS-GLY-ALA-ILE-LEU-THR-THR-MET-LEU-ALA-OH

Biological Activity

[Description]:

Calmodulin-Dependent Protein Kinase II (290-309) is a potent CaMK antagonist with an IC50 of 52 nM for inhibition of Ca2+/calmodulin-dependent protein kinase II.

[Related Catalog]:

Signaling Pathways >> Neuronal Signaling >> CaMK

Research Areas >> Neurological Disease

Peptides

[Target]

IC50: 52 nM (calmodulin-dependent protein kinase II)[1]

[In Vitro]

Peptide 290 to 309 is found to be a potent calmodulin antagonist with an IC50 of 52 nM for inhibition of Ca2+/calmodulin-dependent protein kinase. Neither truncation from the amino terminus (peptide 296-309) nor extension in the carboxyl-terminal direction (peptide 294-319) markedly affects calmodulin binding, whereas shortening the peptide from the carboxyl terminus (peptide 290-302) or from both ends (peptide 295-304) results in the elimination of this activity[1].

[Kinase Assay]

Ca2+/CaM-dependent phosphodiesterase is assayed at 30°C. The reaction mixture contains 40 mM Tris-HCl, pH 8.0, 5 mM magnesium acetate, 1 mM calcium chloride, 30 μM cGMP, 0.15 pCi of [3H]cGMP, 1 mM dithiothreitol, 20% glycerol, 0.64 mg/mL bovine serum albumin, 2.38 nM CaM, 50 pM CaM-deficient phosphodiesterase, and various concentrations of Calmodulin-Dependent Protein Kinase II (290-309). Following preincubation of the reaction mixture at 30°C, the reaction is initiated by the addition of substrate. After 50 min the reaction is terminated by boiling. Conditions are selected in which the reactions are linear with respect to time[1].

[References]

[1]. Payne ME, et al. Calcium/calmodulin-dependent protein kinase II. Characterization of distinct calmodulin binding and inhibitory domains. J Biol Chem. 1988 May 25;263(15):7190-5.

[Related Small Molecules]

KN-93 | STO609 | KN-62 | KN-92-hydrochloride | CaMKII-IN-1 | MLCK inhibitor peptide 18 | Autocamtide-2 | A 484954 | Rimacalib | Metofenazate | NH 125 | Syntide 2 trifluoroacetate salt

Chemical & Physical Properties

[ Molecular Formula ]:
C₁₀₃H₁₈₅N₃₁O₂₄S

[ Molecular Weight ]:
2273.83000

[ Exact Mass ]:
2272.39000

[ PSA ]:
952.95000

[ LogP ]:
7.21720

[ Storage condition ]:
2-8℃

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Safety Phrases ]:
22-24/25

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

Articles

Long-term potentiation is associated with an increased activity of Ca2+/calmodulin-dependent protein kinase II.

J. Biol. Chem. 268 , 7863, (1993)

Among the molecular mechanisms that have been proposed to contribute to long-term potentiation in hippocampus are the activation and autophosphorylation of Ca2+/calmodulin-dependent protein kinase II ...

Activation of calcium channels by cAMP in STC-1 cells is dependent upon Ca2+ calmodulin-dependent protein kinase II.

Biochem. Biophys. Res. Commun. 254 , 699, (1999)

Activation of L-type calcium channels in the neuroendocrine, cholecytstokinin-secreting cell line, STC-1, is vital for secretion of CCK. In the present study, the regulation of L-type Ca2+ channels by...