Indian Journal of Experimental Biology 2011-04-01

Comparative study of 6-APA production by free and agar immobilized bacteria in nutrient broth culture.

A K Dolui, S Das

Index: Indian J. Exp. Biol. 49(4) , 289-92, (2011)

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Abstract

In the present study different bacterial samples were isolated from soil of different places of Dibrugarh and screened for biotransformation ability to produce 6-Aminopenicillanic acid. Among ten isolated bacterial samples, three gram positive bacterial samples designated as AKDD-2, AKDD-4 and AKDD-6 showed the production of 6-APA from penicillin G. Assessment of production of 6-APA after incubation in penicillin G (2 mg/ml) by three different samples separately in free and agar immobilization state was done by HPLC analysis. Reusability of immobilized cells was found successful up to 14 days.