Biotechnology and Applied Biochemistry 1993-02-01

Immobilization of beta-glucosidase from Penicillium funiculosum on nylon powder.

J Aguado, M D Romero, L Rodríguez

Index: Biotechnol. Appl. Biochem. 17 ( Pt 1) , 49-55, (1993)

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Abstract

beta-Glucosidase from Penicillium funiculosum was immobilized on nylon powder previously activated with triethyloxonium tetrafluoroborate, 1,2-diaminoethane and glutaraldehyde. The activation of the nylon powder and the immobilization processes were studied and optimized for the enzyme and the matrix. A high activity retention (67%) was obtained using the activation and immobilization conditions finally selected.