Bioscience, Biotechnology, and Biochemistry 2006-05-01

Identification of 2-(cysteinyl)amido-2-deoxy-D-galacturonic acid residue from the sheath of Leptothrix cholodnii.

Hiroko Makita, Yuichi Nakahara, Hiroyuki Fukui, Yohei Miyanoiri, Masato Katahira, Hiroko Seki, Minoru Takeda, Jun-Ichi Koizumi

Index: Biosci. Biotechnol. Biochem. 70(5) , 1265-8, (2006)

Full Text: HTML

Abstract

The sheath of Leptothrix cholodnii is a glycoconjugate composed of a polysaccharide and a peptide rich in cysteine. In this study, structural determination of the hydrazinolyzate of the sheath was carried out. Since the hydrazinolyzate is a polysaccharide incorporated with cysteine, it was S-derivatized with a thiol-specific fluorogenic reagent, 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (ABD-F). Fluorescent fragments were purified by HPLC, and their structures were analyzed by mass spectrometry and NMR spectroscopy. The sheath was found to contain 2-(cysteinyl)amido-2-deoxy-D-galacturonic acid residue.