Journal of Pharmaceutical and Biomedical Analysis 2015-08-10

Identification of human basic fetoprotein as glucose-6-phosphate isomerase by using N- and C-terminal sequence tags and terminal tag database.

Hiroki Kuyama, Akiyasu C Yoshizawa, Chihiro Nakajima, Mutsumi Hosako, Koichi Tanaka

Index: J. Pharm. Biomed. Anal. 112 , 116-25, (2015)

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Abstract

Human basic fetoprotein (BFP), found in fetal serum and tissue extracts as well as in extracts of various cancer tissues, has long been known as a marker protein for cancers; however, the primary sequence has not yet been reported. This paper describes the identification of BFP using the N- and C-terminal amino acid sequence tags (Ac-AALTRDPQFQ and QQREARVQ, respectively) clarified by mass spectrometry-based methods, and a terminal tag database (ProteinCarta). In this study, BFP was identified as glucose-6-phosphate isomerase (G6PI_HUMAN). Copyright © 2015 Elsevier B.V. All rights reserved.