Advanced Healthcare Materials 2015-08-26

Covalent Incorporation of Trehalose within Hydrogels for Enhanced Long-Term Functional Stability and Controlled Release of Biomacromolecules.

Timothy M O'Shea, Matthew J Webber, Alex A Aimetti, Robert Langer

Index: Adv. Healthc. Mater. 4 , 1802-12, (2015)

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Abstract

Hydrogels with covalently incorporated trehalose are synthesized using thiol-ene Michael addition. Trehalose hydrogels afford prolonged stabilization and -controlled release of model enzymes in vitro and in vivo as well as preservation of protein stability under heat and -lyophilization stressors. Strong and -ordered hydrogen bonding interactions within covalently incorporated trehalose hydrogels represent a possible mechanism for protein stabilization. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.