Synthesis of 2′??deoxynucleosides by transglycosylation with new immobilized and stabilized uridine phosphorylase and purine nucleoside phosphorylase

…, AM Albertini, R Fernández??Lafuente…

Index: Ubiali, Daniela; Rocchietti, Silvia; Scaramozzino, Francesca; Terreni, Marco; Albertini, Alessandra M.; Fernandez-Lafuente, Roberto; Guisan, Jose Manuel; Pregnolato, Massimo Advanced Synthesis and Catalysis, 2004 , vol. 346, # 11 p. 1361 - 1366

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Citation Number: 42

Abstract

Abstract Multimeric uridine phosphorylase (UP) and purine nucleoside phosphorylase (PNP) of Bacillus subtilis have been expressed from genes cloned in Escherichia coli, purified, characterized, immobilized and stabilized on solid support. A new immobilization strategy has been developed for UP onto Sepabeads coated with polyethyleneamine followed by cross-linking with aldehyde-dextran. PNP has been immobilized onto glyoxyl- ...