Synthesis of 2′??deoxynucleosides by transglycosylation with new immobilized and stabilized uridine phosphorylase and purine nucleoside phosphorylase

…, AM Albertini, R Fernández??Lafuente…

文献索引：Ubiali, Daniela; Rocchietti, Silvia; Scaramozzino, Francesca; Terreni, Marco; Albertini, Alessandra M.; Fernandez-Lafuente, Roberto; Guisan, Jose Manuel; Pregnolato, Massimo Advanced Synthesis and Catalysis, 2004 , vol. 346, # 11 p. 1361 - 1366

全文：HTML全文

被引用次数: 42

摘要

Abstract Multimeric uridine phosphorylase (UP) and purine nucleoside phosphorylase (PNP) of Bacillus subtilis have been expressed from genes cloned in Escherichia coli, purified, characterized, immobilized and stabilized on solid support. A new immobilization strategy has been developed for UP onto Sepabeads coated with polyethyleneamine followed by cross-linking with aldehyde-dextran. PNP has been immobilized onto glyoxyl- ...