2-Nitrophenyl β-D-glucopyranoside
2-Nitrophenyl β-D-glucopyranoside structure
|
Common Name | 2-Nitrophenyl β-D-glucopyranoside | ||
|---|---|---|---|---|
| CAS Number | 2816-24-2 | Molecular Weight | 301.249 | |
| Density | 1.6±0.1 g/cm3 | Boiling Point | 572.4±50.0 °C at 760 mmHg | |
| Molecular Formula | C12H15NO8 | Melting Point | N/A | |
| MSDS | USA | Flash Point | 300.0±30.1 °C | |
|
Improving low-temperature catalysis in the hyperthermostable Pyrococcus furiosus beta-glucosidase CelB by directed evolution.
Biochemistry 39(13) , 3656-65, (2000) The beta-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus (CelB) is the most thermostable and thermoactive family 1 glycosylhydrolase described to date. To obtain more insight in the molecular determinants of adaptations to high temperature... |
|
|
Restoration of the activity of active-site mutants of the hyperthermophilic beta-glycosidase from Sulfolobus solfataricus: dependence of the mechanism on the action of external nucleophiles.
Biochemistry 37(49) , 17262-70, (1998) The beta-glycosidase from the hyperthermophilic Archaeon Sulfolobus solfataricus hydrolyzes beta-glycosides following a retaining mechanism based upon the action of two amino acids: Glu387, which acts as the nucleophile of the reaction, and Glu206, which acts... |
|
|
beta-Glucosidase as a reporter for the gene expression studies in Thermus thermophilus and constitutive expression of DNA repair genes.
Mutagenesis 21(4) , 255-60, (2006) Thermus thermophilus is an extremely thermophilic eubacterium that grows optimally at 70-75 degrees C. Because the frequency of DNA damage, such as deamination, depurination and single-strand breaks, increases as the temperature rises, the regulation of expre... |
|
|
Highly productive autocondensation and transglycosylation reactions with Sulfolobus solfataricus glycosynthase.
ChemBioChem. 6(8) , 1431-7, (2005) Transglycosylation reactions (autocondensation of the substrate or transfer of the glycon donor moiety to different acceptors) with the hyperthermophilic glycosynthase from Sulfolobus solfataricus acting in dilute sodium formate buffer at pH 4.0 are reported;... |
|
|
Characterization of two membrane-associated beta-glucosidases from maize (Zea mays L.) coleoptiles.
Biochem. J. 302 ( Pt 1) , 15-21, (1994) We isolated membrane vesicles from maize (Zea mays L.) coleoptiles and identified in these vesicles a 58 kDa (pm58) and a 60 kDa (pm60) protein by photoaffinity labelling with 5-azido-[7-3H]indole-3-acetic acid ([3H]N3IAA). Photoaffinity labelling was effecti... |
|
|
Detection of inactive precursors of beta-glucanases in Saccharomyces cerevisiae.
FEBS Lett. 161(2) , 190-4, (1983) Accumulation and secretion of beta-glucanases have been studied in vivo by using a thermosensitive secretory mutant of Saccharomyces cerevisiae blocked at the endoplasmic reticulum level (sec 18-1). When incubated at the restrictive temperature no accumulatio... |