Phenylalanine,N-acetyl-, 2-naphthalenyl ester
Phenylalanine,N-acetyl-, 2-naphthalenyl ester structure
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Common Name | Phenylalanine,N-acetyl-, 2-naphthalenyl ester | ||
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| CAS Number | 20874-31-1 | Molecular Weight | 333.38000 | |
| Density | 1.2g/cm3 | Boiling Point | 589.8ºC at 760mmHg | |
| Molecular Formula | C21H19NO3 | Melting Point | N/A | |
| MSDS | USA | Flash Point | 310.5ºC | |
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A simple method to determine trypsin and chymotrypsin inhibitory activity.
J. Biochem. Biophys. Methods 59 , 241-251, (2004) A colorimetric method for serine protease inhibition was modified using N-Acetyl-DL-Phenylalanine beta-Naphthylester (APNE) as the substrate and o-Dianisidine tetrazotized (oD) as the dye. The reaction generated a single peak absorbing at 530 nm for both tryp... |
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An association between Schistosoma mansoni worms and an enzymatically-active protease/peptidase in mouse blood.
Parasitology 135 , 467-472, (2008) An enzyme found previously in extracts of adult Schistosoma mansoni worms, that hydrolysed the chromogenic substrate N-acetyl-DL-phenylalanine beta-naphthyl-ester, has here been further investigated and characterized. Evidence that the molecule found in the p... |
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Involvement of proteases in the action of IFN-gamma on WISH cells.
J. Interferon Cytokine Res. 22(8) , 847-52, (2002) This study investigates the possible involvement of serine proteases in interferon-gamma (IFN-gamma) activity on WISH cells. It was observed that inhibition of (3)H-thymidine incorporation induced by IFN-gamma was abrogated by the serine protease inhibitors N... |
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Assay for enzyme inhibition: detection of natural inhibitors of trypsin and chymotrypsin.
Anal. Biochem. 162 , 345, (1987) A technique for quickly detecting nanogram quantities of low- and high-molecular-weight inhibitors of some serine proteases is described. The inhibitor solutions are spotted onto agar films which contain either L-1-p-tosylamino-2-phenylethyl chloromethyl keto... |
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Electrophoretic separation of proteolytic enzymes in pancreatic juice collected with the pouch or catheter method.
Int. J. Pancreatol. 22 , 39-43, (1997) The results of this study demonstrated that proteolytic enzymes in pancreatic juice from pigs prepared with the pouch method (PM) were nearly fully active or were fully active. When activation with enterokinase was carried out further inactivation and/or brea... |
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Quantitative determination of serine proteinase inhibitor activity using a radial diffusion assay.
Anal. Biochem. 212 , 79-84, (1993) An improved, time efficient, visual assay for quantitative determination of proteinase inhibitor activity in protein extracts is reported. Proteinase inhibitor activity of mammalian, bacterial, and fungal serine proteinases can be quantified. The method relie... |
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Schistosoma mansoni: anomalous immunogenic properties of a 27 kDa larval serine protease associated with protective immunity.
Parasitology 115 , 237-247, (1997) A cationic Schistosoma mansoni cercarial antigen was shown to be a serine protease as it was capable of hydrolysing N-acetyl-DL-phenylalanine beta-naphthyl ester (NAPBNE) after precipitation by immunoelectrophoresis, and this reaction was modulated by the ser... |
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Cytochemical determination of granulocyte elastase and chymotrypsin in human myeloid cells and its application in acquired deficiency states and diagnosis of myeloid leukemia.
Klin. Wochenschr. 61(1) , 49-56, (1983) Two cytochemical methods for detection of granulocytic elastase and chymotrypsin employing alanine and phenylalanine naphthyl esters were developed. Specificity of reaction with the ester substrates was proven by chloromethyl ketone inhibitors. The results of... |
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Mutations affecting a regulated, membrane-associated esterase in Salmonella typhimurium LT2.
Mol. Gen. Genet. 243(6) , 674-80, (1994) Mutations at the apeA locus in Salmonella typhimurium lead to loss of a soluble enzyme ("protease I") that hydrolyzes the chromogenic endoprotease substrate N-acetyl phenylalanine beta-naphthyl ester. We have isolated pseudorevertants of S. typhimurium apeA m... |