5-methyl-2-thiouridine
5-methyl-2-thiouridine structure
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Common Name | 5-methyl-2-thiouridine | ||
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| CAS Number | 32738-09-3 | Molecular Weight | 274.29 | |
| Density | 1.63g/cm3 | Boiling Point | N/A | |
| Molecular Formula | C10H14N2O5S | Melting Point | 216-219 °C | |
| MSDS | Chinese USA | Flash Point | N/A | |
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Molecular mechanism of codon recognition by tRNA species with modified uridine in the first position of the anticodon.
Proc. Natl. Acad. Sci. U. S. A. 82 , 4905-4909, (1985) Proton NMR analyses have been made to elucidate the conformational characteristics of modified nucleotides as found in the first position of the anticodon of tRNA [derivatives of 5-methyl-2-thiouridine 5'-monophosphate (pxm5s2U) and derivatives of 5-hydroxyur... |
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CD spectra of 5-methyl-2-thiouridine in tRNA-Met-f from an extreme thermophile.
Nucleic Acids Res. 3 , 1703-1713, (1976) 5-Methyl-2-thiouridine (S) in tRNA-Met-f from an extreme thermophile is located in the TpsiC region, replacing T, and has a positive CD band centered at 310 nm. Upon heating, the profiles of the change in this band were similar to the UV melting profiles of t... |
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Stabilizing contributions of sulfur-modified nucleotides: crystal structure of a DNA duplex with 2'-O-[2-(methoxy)ethyl]-2-thiothymidines.
Nucleic Acids Res. 33 , 5297-5307, (2005) Substitution of oxygen atoms by sulfur at various locations in the nucleic acid framework has led to analogs such as the DNA phosphorothioates and 4'-thio RNA. The phosphorothioates are excellent mimics of DNA, exhibit increased resistance to nuclease degrada... |
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NMR analyses on the molecular mechanism of the conformational rigidity of 2-thioribothymidine, a modified nucleoside in extreme thermophile tRNAs.
FEBS Lett. 157(1) , 95-9, (1983) 1H-NMR analyses have been made on the conformations of 2-thioribothymidine (s2T), 2-thiodeoxyribothymidine (s2dT), as well as ribothymidine (T) and deoxyribothymidine (dT). s2T and s2dT exclusively take the anti form rather than the syn form. The C3'-endo-gg ... |
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Temperature-dependent biosynthesis of 2-thioribothymidine of Thermus thermophilus tRNA.
J. Biol. Chem. 281(4) , 2104-13, (2006) 2-Thioribothymidine (s(2)T) is a modified nucleoside of U, specifically found at position 54 of tRNAs from extreme thermophilic microorganisms. The function of the 2-thiocarbonyl group of s(2)T54 is thermostabilization of the three-dimensional structure of tR... |
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Two tRNAIle1 species from an extreme thermophile, Thermus thermophilus HB8: effect of 2-thiolation of ribothymidine on the thermostability of tRNA.
Biochemistry 24(21) , 5711-5, (1985) From Thermus thermophilus HB8 grown at 65 degrees C, two major tRNAIle species have been purified by column chromatography and polyacrylamide gel electrophoresis. The nucleotide sequence of one of these two tRNAIle1 species (tRNAIle1a) has been determined to ... |
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Circular dichroism analyses of tRNA X protein interactions.
Nucleic Acids Symp. Ser. (17) , 199-201, (1986) The interactions of tRNA species with aminoacyl-tRNA synthetases and polypeptide chain elongation factor Tu from Thermus thermophilus HB8 were studied by the analyses mainly of the circular dichroism band of 2-thioribothymidine in position 54 of T. thermophil... |
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Separation and comparison of 2-thioribothymidine-containing transfer ribonucleic acid and the ribothymidine-containing counterpart from cells of Thermus thermophilus HB 8.
Biochemistry 22(1) , 98-102, (1983) For the extreme thermophile Thermus thermophilus HB 8, a positive correlation was observed among the growth temperatures of the cells, the melting temperature, and the 2-thioribothymidine (s2T) content of tRNA extracted from cells grown at various temperature... |
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Selective utilization of 2-thioribothymidine- and ribothymidine-containing tRNAs by the protein synthetic systems of Thermus thermophilus HB 8 depending on the environmental temperature.
J. Biochem. 96(5) , 1625-32, (1984) An extreme thermophile, Thermus thermophilus HB 8, contains two types of tRNAs, T- and S2T-containing tRNAs. Their relative content changes depend on the growth temperature of the bacterial cells (1-3). To elucidate the reason why the extreme thermophile poss... |
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Reactions of 2-thioribothymidine ad 4-thiouridine with hydrogen peroxide in transfer ribonucleic acids from Thermus thermophilus and Escherichia coli as studied by circular dichroism.
Biochemistry 19(24) , 5542-9, (1980)
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