EMA [Ethidium monoazide bromide]

EMA [Ethidium monoazide bromide] Structure
EMA [Ethidium monoazide bromide] structure
Common Name EMA [Ethidium monoazide bromide]
CAS Number 58880-05-0 Molecular Weight 420.30500
Density N/A Boiling Point N/A
Molecular Formula C21H18BrN5 Melting Point N/A
MSDS Chinese USA Flash Point N/A

Modified vaccinia virus Ankara-infected dendritic cells present CD4+ T-cell epitopes by endogenous major histocompatibility complex class II presentation pathways.

J. Virol. 89(5) , 2698-709, (2015)

CD4(+) T lymphocytes play a central role in the immune system and mediate their function after recognition of their respective antigens presented on major histocompatibility complex II (MHCII) molecules on antigen-presenting cells (APCs). Conventionally, phag...

Propidium monoazide (PMA) and ethidium bromide monoazide (EMA) improve DNA array and high-throughput sequencing of porcine reproductive and respiratory syndrome virus identification.

J. Virol. Methods 222 , 182-91, (2015)

Pan-viral DNA array (PVDA) and high-throughput sequencing (HTS) are useful tools to identify novel viruses of emerging diseases. However, both techniques have difficulties to identify viruses in clinical samples because of the host genomic nucleic acid conten...

Extracellular matrices decellularized from embryonic stem cells maintained their structure and signaling specificity.

Tissue Eng. Part A 20(1-2) , 54-66, (2014)

Embryonic stem cells (ESCs) emerge as a promising tool for tissue engineering and regenerative medicines due to their extensive self-renewal ability and the capacity to give rise to cells from all three-germ layers. ESCs also secrete a large amount of endogen...

Antigen targeting to dendritic cells combined with transient regulatory T cell inhibition results in long-term tumor regression.

Oncoimmunology 4 , e970462, (2015)

Therapeutic vaccinations against cancer are still largely ineffective. Major caveats are inefficient delivery of tumor antigens to dendritic cells (DCs) and excessive immune suppression by Foxp3(+) regulatory T cells (Tregs), resulting in defective T cell pri...

Kindlin-3-mediated integrin adhesion is dispensable for quiescent but essential for activated hematopoietic stem cells.

J. Exp. Med. 212 , 1415-32, (2015)

Hematopoietic stem cells (HSCs) generate highly dividing hematopoietic progenitor cells (HPCs), which produce all blood cell lineages. HSCs are usually quiescent, retained by integrins in specific niches, and become activated when the pools of HPCs decrease. ...

Assessment of Ethidium bromide and Ethidium monoazide bromide removal from aqueous matrices by adsorption on cupric oxide nanoparticles

Ecotoxicol. Environ. Saf. 104 , 386-92, (2014)

The present study was undertaken to develop an effective adsorbent and to study the adsorption of Ethidium bromide and Ethidium monoazide bromide from aqueous solution using the CuO nanoparticles. The characteristics of CuO nanoparticles were determined and f...

Real time PCR quantification of viable Mycobacterium tuberculosis from sputum samples treated with propidium monoazide.

Tuberculosis (Edinb.) 94(4) , 421-7, (2014)

Diagnostic methods of TB, nowadays, are prone to delay in diagnosis, increased false negative results and are not sensitive to many forms of paucibacillary disease. The aims of this study were to implement a quantitative nucleic acid-based diagnostic test for...

A bacteria deriving peptide modified dendrigraft poly-l-lysines (DGL) self-assembling nanoplatform for targeted gene delivery.

Mol. Pharm. 11(10) , 3330-41, (2014)

Achieving effective gene therapy for glioma depends on gene delivery systems. The gene delivery system should be able to cross the blood-brain barrier (BBB) and further target glioma at its early stage. Active brain tumor targeted delivery can be achieved usi...

The effect of various environmental factors on the ethidium monazite and quantitative PCR method to detect viable bacteria.

J. Appl. Microbiol. 111(5) , 1194-204, (2011)

Ethidium monoazide in combination with quantitative PCR (EMA-qPCR) has been considered as a promising method to enumerate viable cells; however, its efficacy can be significantly affected by disinfection conditions and various environments. In this study, the...

New approach to use ethidium bromide monoazide as an analytical tool.

J. Appl. Microbiol. 109(3) , 900-9, (2010)

Ethidium bromide monoazide (EMA) has been determined to cause delay in DNA amplification from dead bacteria at real-time PCR. However, there is concern that the increasing EMA concentration to suppress amplification from high number of dead bacteria also affe...