2-Phenylethyl β-D-thiogalactoside

2-Phenylethyl β-D-thiogalactoside Structure
2-Phenylethyl β-D-thiogalactoside structure
Common Name 2-Phenylethyl β-D-thiogalactoside
CAS Number 63407-54-5 Molecular Weight 300.37100
Density 1.39 g/cm3 Boiling Point 539.7ºC at 760 mmHg
Molecular Formula C14H20O5S Melting Point 100ºC
MSDS USA Flash Point 280.2ºC

Profiling of the Chromatin-associated Proteome Identifies HP1BP3 as a Novel Regulator of Cell Cycle Progression.

Mol. Cell. Proteomics 0 , (2014)

The chromatin-associated proteome (chromatome) regulates cellular gene expression by restricting access of transcriptional machinery to template DNA, and dynamic re-modeling of chromatin structure is required to regulate critical cell functions including grow...

Thermal stability of purified and reconstituted CFTR in a locked open channel conformation.

Protein Expr. Purif. 116 , 159-66, (2015)

CFTR is unique among ABC transporters as the only one functioning as an ion channel and from a human health perspective because mutations in its gene cause cystic fibrosis. Although considerable advances have been made towards understanding CFTR's mechanism o...

Long-term imaging of Caenorhabditis elegans using nanoparticle-mediated immobilization.

PLoS ONE 8(1) , e53419, (2013)

One advantage of the nematode Caenorhabditis elegans as a model organism is its suitability for in vivo optical microscopy. Imaging C. elegans often requires animals to be immobilized to avoid movement-related artifacts. Immobilization has been performed by a...

High-resolution dose-response screening using droplet-based microfluidics.

Proc. Natl. Acad. Sci. U. S. A. 109 , 378-83, (2012)

A critical early step in drug discovery is the screening of a chemical library. Typically, promising compounds are identified in a primary screen and then more fully characterized in a dose-response analysis with 7-10 data points per compound. Here, we descri...

Integration of on-column immobilized enzyme reactor in microchip electrophoresis.

Electrophoresis 24 , 200-6, (2003)

A simple method integrating an immobilized enzyme reactor into a microchip electrophoresis device was developed. The enzyme immobilization into a microchip was performed by spotting and drying a drop of dissolved nitrocellulose (NC) on a glass substrate, and ...

Subnanoliter enzymatic assays on microarrays.

Proteomics 5 , 420-5, (2005)

Many areas of research today are based on enzymatic assays most of which are still performed as enzyme-linked immunosorbent assays in microtiter plates. The demand for highly parallel screening of thousands of samples eventually led to a miniaturization and a...

Improved FACS-Gal: flow cytometric analysis and sorting of viable eukaryotic cells expressing reporter gene constructs.

Cytometry 12 , 291, (1991)

The previously reported FACS-Gal assay (Nolan et al., Proc Natl Acad Sci USA 85:2603-2607, 1988) measures E. coli lacZ-encoded beta-galactosidase activity in individual viable eukaryotic cells for a variety of molecular and cellular biological applications. E...

Key features of σS required for specific recognition by Crl, a transcription factor promoting assembly of RNA polymerase holoenzyme.

Proc. Natl. Acad. Sci. U. S. A. 110(40) , 15955-60, (2013)

Bacteria use multiple sigma factors to coordinate gene expression in response to environmental perturbations. In Escherichia coli and other γ-proteobacteria, the transcription factor Crl stimulates σ(S)-dependent transcription during times of cellular stress ...

The nuclear effector of Wnt-signaling, Tcf1, functions as a T-cell-specific tumor suppressor for development of lymphomas.

PLoS Biol. 10 , e1001430, (2012)

The HMG-box factor Tcf1 is required during T-cell development in the thymus and mediates the nuclear response to Wnt signals. Tcf1(-/-) mice have previously been characterized and show developmental blocks at the CD4-CD8- double negative (DN) to CD4+CD8+ doub...

Thermal denaturation of beta-galactosidase and of two site-specific mutants.

Biochemistry 29(49) , 11001-8, (1990)

The thermal denaturation of wild-type beta-galactosidase and two beta-galactosidases with substitutions at the active site was studied by kinetics, differential scanning calorimetry, electrophoresis, molecular exclusion chromatography, and circular dichroism....