Restriction Endonuclease BstEⅡ
Restriction Endonuclease BstEⅡ structure
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Common Name | Restriction Endonuclease BstEⅡ | ||
|---|---|---|---|---|
| CAS Number | 93229-61-9 | Molecular Weight | N/A | |
| Density | N/A | Boiling Point | N/A | |
| Molecular Formula | N/A | Melting Point | N/A | |
| MSDS | USA | Flash Point | N/A | |
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Double-strand break repair by homologous recombination in primary mouse somatic cells requires BRCA1 but not the ATM kinase.
Proc. Natl. Acad. Sci. U. S. A. 110(14) , 5564-9, (2013) Homology-directed repair (HDR) is a critical pathway for the repair of DNA double-strand breaks (DSBs) in mammalian cells. Efficient HDR is thought to be crucial for maintenance of genomic integrity during organismal development and tumor suppression. However... |
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Helicobacter pylori DprA alleviates restriction barrier for incoming DNA.
Nucleic Acids Res. 41(5) , 3274-88, (2013) Helicobacter pylori is a Gram-negative bacterium that colonizes human stomach and causes gastric inflammation. The species is naturally competent and displays remarkable diversity. The presence of a large number of restriction-modification (R-M) systems in th... |
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Palindromic sequence impedes sequencing-by-ligation mechanism.
BMC Syst. Biol. 6 Suppl 2 , S10, (2012) Current next-generation sequencing (NGS) platforms adopt two types of sequencing mechanisms: by synthesis or by ligation. The former is employed by 454 and Solexa systems, while the latter by SOLiD system. Although the pros and cons for each sequencing mechan... |
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Conserved DNA methylation patterns in healthy blood cells and extensive changes in leukemia measured by a new quantitative technique.
Epigenetics 7(12) , 1368-78, (2012) Genome wide analysis of DNA methylation provides important information in a variety of diseases, including cancer. Here, we describe a simple method, Digital Restriction Enzyme Analysis of Methylation (DREAM), based on next generation sequencing analysis of m... |
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rDNA-directed integration by an HIV-1 integrase--I-PpoI fusion protein.
Nucleic Acids Res. 41(5) , e61, (2013) Integrating viral vectors are efficient gene transfer tools, but their integration patterns have been associated with genotoxicity and oncogenicity. The recent development of highly specific designer nucleases has enabled target DNA modification and site-spec... |
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Allosteric regulation of DNA cleavage and sequence-specificity through run-on oligomerization.
Structure 21(10) , 1848-58, (2013) SgrAI is a sequence specific DNA endonuclease that functions through an unusual enzymatic mechanism that is allosterically activated 200- to 500-fold by effector DNA, with a concomitant expansion of its DNA sequence specificity. Using single-particle transmis... |
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Harnessing mutagenic homologous recombination for targeted mutagenesis in vivo by TaGTEAM.
Nucleic Acids Res. 41(9) , e99, (2013) A major hurdle to evolutionary engineering approaches for multigenic phenotypes is the ability to simultaneously modify multiple genes rapidly and selectively. Here, we describe a method for in vivo-targeted mutagenesis in yeast, targeting glycosylases to emb... |
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The MASTER (methylation-assisted tailorable ends rational) ligation method for seamless DNA assembly.
Nucleic Acids Res. 41(8) , e93, (2013) Techniques for assembly of designed DNA sequences are important for synthetic biology. So far, a few methods have been developed towards high-throughput seamless DNA assembly in vitro, including both the homologous sequences-based system and the type IIS-medi... |
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Transient systemic mtDNA damage leads to muscle wasting by reducing the satellite cell pool.
Hum. Mol. Genet. 22(19) , 3976-86, (2013) With age, muscle mass and integrity are progressively lost leaving the elderly frail, weak and unable to independently care for themselves. Defined as sarcopenia, this age-related muscle atrophy appears to be multifactorial but its definite cause is still unk... |
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IgH class switching exploits a general property of two DNA breaks to be joined in cis over long chromosomal distances.
Proc. Natl. Acad. Sci. U. S. A. 111(7) , 2644-9, (2014) Antibody class switch recombination (CSR) in B lymphocytes joins two DNA double-strand breaks (DSBs) lying 100-200 kb apart within switch (S) regions in the immunoglobulin heavy-chain locus (IgH). CSR-activated B lymphocytes generate multiple S-region DSBs in... |