Ribonuclease T1 from Aspergillus oryzae(Chromatographically Purif.)

Ribonuclease T1 from Aspergillus oryzae(Chromatographically Purif.) Structure
Ribonuclease T1 from Aspergillus oryzae(Chromatographically Purif.) structure
Common Name Ribonuclease T1 from Aspergillus oryzae(Chromatographically Purif.)
CAS Number 9026-12-4 Molecular Weight 384.41
Density N/A Boiling Point N/A
Molecular Formula N/A Melting Point N/A
MSDS USA Flash Point N/A

Template-directed ligation of tethered mononucleotides by t4 DNA ligase for kinase ribozyme selection.

PLoS ONE 5(8) , e12368, (2010)

In vitro selection of kinase ribozymes for small molecule metabolites, such as free nucleosides, will require partition systems that discriminate active from inactive RNA species. While nucleic acid catalysis of phosphoryl transfer is well established for pho...

FMRP mediates mGluR5-dependent translation of amyloid precursor protein.

PLoS Biol. 5 , e52, (2007)

Amyloid precursor protein (APP) facilitates synapse formation in the developing brain, while beta-amyloid (Abeta) accumulation, which is associated with Alzheimer disease, results in synaptic loss and impaired neurotransmission. Fragile X mental retardation p...

A biphasic pattern of gene expression during mouse retina development.

BMC Dev. Biol. 6 , 48, (2006)

Between embryonic day 12 and postnatal day 21, six major neuronal and one glia cell type are generated from multipotential progenitors in a characteristic sequence during mouse retina development. We investigated expression patterns of retina transcripts duri...

Electrically-assisted delivery of an anionic protein across intact skin: cathodal iontophoresis of biologically active ribonuclease T1.

J. Control. Release 152(3) , 356-62, (2011)

Cathodal iontophoresis of anionic macromolecules has been considered a major challenge owing to (i) the presence of a negative charge on the skin under physiological conditions and (ii) the electroosmotic solvent flow in the (opposite) anode-to-cathode direct...

Reaction of singlet oxygen with tryptophan in proteins: a pronounced effect of the local environment on the reaction rate.

J. Am. Chem. Soc. 134(23) , 9820-6, (2012)

Singlet molecular oxygen, O(2)(a(1)Δ(g)), can influence many processes pertinent to the function of biological systems, including events that result in cell death. Many of these processes involve a reaction between singlet oxygen and a given amino acid in a p...

Structure of long human telomeric RNA (TERRA): G-quadruplexes formed by four and eight UUAGGG repeats are stable building blocks.

Biochemistry 50(29) , 6455-61, (2011)

The discovery of long RNA transcripts of telomeric repeats (TERRA) and their potential to form G-quadruplexes stimulated studies on the possible arrangements of G-quadruplexes along TERRA. Here we performed ribonuclease protection assay to investigate the str...

RNAse mapping and quantitation of RNA isoforms.

Methods Mol. Biol. 883 , 121-9, (2012)

The ribonuclease protection assay (RPA) has emerged as an important methodology for the detection, mapping, and quantification of RNAs. In this assay, total or cytoplasmic RNAs are hybridized to a high-specific activity antisense radioactive RNA probe synthes...

Nonenzymatic oligomerization of activated nucleotides on hairpin templates.

Curr. Protoc. Nucleic Acid Chem. Chapter 3 , Unit3.18, (2009)

This unit describes a protocol for nonenzymatic oligomerization of activated ribonucleotides on DNA hairpins appended by templates containing threofuranosyl nucleic acid (TNA). TNA-cytidylate templates effectively promote oligomerization of 2-MeImpG, and give...

Transcriptome-wide identification of A > I RNA editing sites by inosine specific cleavage.

RNA 19(2) , 257-70, (2013)

Adenosine to inosine (A > I) RNA editing, which is catalyzed by the ADAR family of proteins, is one of the fundamental mechanisms by which transcriptomic diversity is generated. Indeed, a number of genome-wide analyses have shown that A > I editing is not lim...

A polyhedron made of tRNAs.

Nature Chemistry 2(9) , 772-9, (2010)

Supramolecular assembly is a powerful strategy used by nature to build nanoscale architectures with predefined sizes and shapes. With synthetic systems, however, numerous challenges remain to be solved before precise control over the synthesis, folding and as...