Abstract 2′-Deoxyadenosine, 2′-deoxycytidine, 2′-deoxyguanosine and 2′- deoxyuridine were prepared with stereoselective deuteriation at C-5′ and used to assign the prochiral C-5′ protons in 300 MHz 1 H NMR spectra obtained in 2 H 2 O. In all cases, the more shielded C-5′ proton was found to be the pro-R proton. From these assignments, C-4′–C-5′ rotamer populations were determined using three previously published ...
