| Description |
ISX-1 is an isoxazole. ISX-1 has anti-adipogenic and pro-osteogenic activities. ISX-1 can be used for the research of osteopenia and osteoporosis[1].
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| Related Catalog |
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| Target |
IC50: 1.9 μM (lipid droplet formation); EC50: 1.2 μM (induce ALP)[1]
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| In Vitro |
ISX-1 剂量依赖性地抑制细胞内脂滴的积累并刺激 ALP 活性[1]。 ISX-1 可形成脂滴，IC50 值为 1.9 μM，也可诱导 ALP，EC50 值为 1.2 μM[1]。 ISX-1（0、1.3、6.5 和 33 μM；3 天）抑制 hBMSCs 成脂分化过程中 PPARγ 和 FABP4 基因的 mRNA 诱导[1]。 ISX-1（0、1.3、6.5 和 33 μM）促进 TCF/LEF 介导的基因转录[1]。 RT-PCR[1] Cell Line: Human bone marrow mesenchymal stem cells (hBMSCs) Concentration: 0, 1.3, 6.5 and 33 μM Incubation Time: 3 days Result: Inhibited the mRNA induction of PPARγ and FABP4 genes under the adipogenic differentiation of hBMSCs and showed no effect on C/EBPα expression. Western Blot Analysis[1] Cell Line: HEK 293 cells Concentration: 6.5 and 33 μM Incubation Time: 6.5 and 33 μM Result: Increased both the amount of activated and total β-catenin.
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| References |
[1]. Nawa, Katsuhiko et al. Discovering small molecules that inhibit adipogenesis and promote osteoblastogenesis: unique screening and Oncostatin M-like activity. Differentiation. 2013 Jul-Sep;86(1-2):65-74.
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