Trypsin

Modify Date: 2025-08-21 15:22:14

Trypsin structure	Common Name	Trypsin
	CAS Number	9002-07-7	Molecular Weight	372.1
	Density	N/A	Boiling Point	N/A
	Molecular Formula	C₆H₁₅O₁₂P₃	Melting Point	115°C
	MSDS	Chinese USA	Flash Point	N/A
	Symbol	GHS07, GHS08	Signal Word	Danger

Use of Trypsin

Trypsin is an enzyme that hydrolyzes proteins at the carboxyl side of the Lysine or Arginine. Trypsin activates PAR2 and PAR4. Trypsin induces cell-to-cell membrane fusion in PDCoV infection by the interaction of S glycoprotein of PDCoV and pAPN. Trypsin also promotes cell proliferation and differentiation. Trypsin can be used in the research of wound healing and neurogenic inflammation[1][2][3][4][6].

Name	Trypsin
Synonym	More Synonyms

Description	Trypsin is an enzyme that hydrolyzes proteins at the carboxyl side of the Lysine or Arginine. Trypsin activates PAR2 and PAR4. Trypsin induces cell-to-cell membrane fusion in PDCoV infection by the interaction of S glycoprotein of PDCoV and pAPN. Trypsin also promotes cell proliferation and differentiation. Trypsin can be used in the research of wound healing and neurogenic inflammation[1][2][3][4][6].
Related Catalog	Research Areas >> Infection Signaling Pathways >> GPCR/G Protein >> Protease-Activated Receptor (PAR) Research Areas >> Inflammation/Immunology
Target	PAR2, PAR4[6]
In Vitro	Trypsin (5 μg/mL, 24 or 48 h) promotes porcine deltacoronavirus (PDCoV) replication in LLC-PK cells[2]. Trypsin (10 and 50 ng/mL, 12 h) enhances PDCoV cell-to-cell spread in LLC-PK cells by promoting membrane fusion in LLC-PK cells[2]. Trypsin (0.05%, 3 h) promotes C6 glioma cell proliferation in serum-free and growth factor-free medium[3]. Trypsin (20 -150 ng/mL, 5 days) potentiates PBMC differentiation[4]. Western Blot Analysis[2] Cell Line: LLC-PK cells, ST cells Concentration: 5 μg/mL Incubation Time: 24 or 48 h Result: Promoted PDCoV replication in LLC-PK cells but not ST cells. Immunofluorescence[2] Cell Line: LLC-PK cells, ST cells Concentration: 10 and 50 ng/mL Incubation Time: 12 h Result: Significantly increased cell-to-cell fusion activity during PDCoV infection of LLC-PK cells.
In Vivo	Trypsin (100-500 μg per site in 50 μL saline, intradermal injection) induces scratching behaviour in mice[5]. Animal Model: Swiss mice[5] Dosage: 100-500 μg per site, in saline (50 μL) Administration: Intradermal injection Result: Induced pruritus, and was inhibited by trypsin inhibitor.
References	[1]. Bhupendra S.Kaphalia. Chapter 16 - Biomarkers of acute and chronic pancreatitis. Biomarkers in Toxicology. 2014, Pages 279-289. [2]. Yue-Lin Yang,et al. Trypsin promotes porcine deltacoronavirus mediating cell-to-cell fusion in a cell type-dependent manner. Emerg Microbes Infect. 2020 Feb 24;9(1):457-468. [3]. H Amano, et al. Trypsin promotes C6 glioma cell proliferation in serum- and growth factor-free medium. Neurosci Res. 1996 Jul;25(3):203-8. [4]. Michael J. V. White, et al. Trypsin Potentiates Human Fibrocyte Differentiation. PLoS One. 2013; 8(8): e70795. [5]. R Costa, et al. Evidence for the role of neurogenic inflammation components in trypsin-elicited scratching behaviour in mice. Br J Pharmacol. 2008 Jul;154(5):1094-103. [6]. F Schmidlin, et al. Protease-activated receptors: how proteases signal to cells. Curr Opin Pharmacol. 2001 Dec;1(6):575-82.

Melting Point	115°C
Molecular Formula	C₆H₁₅O₁₂P₃
Molecular Weight	372.1
Appearance of Characters	lyophilized powder
Storage condition	−20°C
Stability	Stable. Incompatible with strong oxidizing agents.
Water Solubility	Reconstitute in aqueous buffer \| Soluble in water (10 mg/ml), phosphate buffers (10 mg/ml), and balanced salt solutions (1 mg/ml).

Symbol	GHS07, GHS08
Signal Word	Danger
Hazard Statements	H315-H319-H334-H335
Precautionary Statements	P261-P305 + P351 + P338-P342 + P311
Personal Protective Equipment	dust mask type N95 (US);Eyeshields;Faceshields;Gloves
Hazard Codes	Xn,B
Risk Phrases	36/37/38-42-42/43
Safety Phrases	22-24-26-36/37-45-23
RIDADR	NONH for all modes of transport
WGK Germany	2
RTECS	GC3050000

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MFCD00082094

EINECS 232-650-8