9002-07-7

9002-07-7 structure

Name: Trypsin
Chemical Name: Trypsin
CAS Number: 9002-07-7
Molecular Formula: C₆H₁₅O₁₂P₃
Molecular Weight: 372.1
Catalog: Biochemical Enzymes and coenzymes
Create Date: 2018-12-27 16:30:57
Modify Date: 2025-08-21 15:22:14
Trypsin is an enzyme that hydrolyzes proteins at the carboxyl side of the Lysine or Arginine. Trypsin activates PAR2 and PAR4. Trypsin induces cell-to-cell membrane fusion in PDCoV infection by the interaction of S glycoprotein of PDCoV and pAPN. Trypsin also promotes cell proliferation and differentiation. Trypsin can be used in the research of wound healing and neurogenic inflammation[1][2][3][4][6].

Name	Trypsin
Synonyms	MFCD00082094 EINECS 232-650-8

Description	Trypsin is an enzyme that hydrolyzes proteins at the carboxyl side of the Lysine or Arginine. Trypsin activates PAR2 and PAR4. Trypsin induces cell-to-cell membrane fusion in PDCoV infection by the interaction of S glycoprotein of PDCoV and pAPN. Trypsin also promotes cell proliferation and differentiation. Trypsin can be used in the research of wound healing and neurogenic inflammation[1][2][3][4][6].
Related Catalog	Research Areas >> Infection Signaling Pathways >> GPCR/G Protein >> Protease-Activated Receptor (PAR) Research Areas >> Inflammation/Immunology
Target	PAR2, PAR4[6]
In Vitro	Trypsin (5 μg/mL, 24 or 48 h) promotes porcine deltacoronavirus (PDCoV) replication in LLC-PK cells[2]. Trypsin (10 and 50 ng/mL, 12 h) enhances PDCoV cell-to-cell spread in LLC-PK cells by promoting membrane fusion in LLC-PK cells[2]. Trypsin (0.05%, 3 h) promotes C6 glioma cell proliferation in serum-free and growth factor-free medium[3]. Trypsin (20 -150 ng/mL, 5 days) potentiates PBMC differentiation[4]. Western Blot Analysis[2] Cell Line: LLC-PK cells, ST cells Concentration: 5 μg/mL Incubation Time: 24 or 48 h Result: Promoted PDCoV replication in LLC-PK cells but not ST cells. Immunofluorescence[2] Cell Line: LLC-PK cells, ST cells Concentration: 10 and 50 ng/mL Incubation Time: 12 h Result: Significantly increased cell-to-cell fusion activity during PDCoV infection of LLC-PK cells.
In Vivo	Trypsin (100-500 μg per site in 50 μL saline, intradermal injection) induces scratching behaviour in mice[5]. Animal Model: Swiss mice[5] Dosage: 100-500 μg per site, in saline (50 μL) Administration: Intradermal injection Result: Induced pruritus, and was inhibited by trypsin inhibitor.
References	[1]. Bhupendra S.Kaphalia. Chapter 16 - Biomarkers of acute and chronic pancreatitis. Biomarkers in Toxicology. 2014, Pages 279-289. [2]. Yue-Lin Yang,et al. Trypsin promotes porcine deltacoronavirus mediating cell-to-cell fusion in a cell type-dependent manner. Emerg Microbes Infect. 2020 Feb 24;9(1):457-468. [3]. H Amano, et al. Trypsin promotes C6 glioma cell proliferation in serum- and growth factor-free medium. Neurosci Res. 1996 Jul;25(3):203-8. [4]. Michael J. V. White, et al. Trypsin Potentiates Human Fibrocyte Differentiation. PLoS One. 2013; 8(8): e70795. [5]. R Costa, et al. Evidence for the role of neurogenic inflammation components in trypsin-elicited scratching behaviour in mice. Br J Pharmacol. 2008 Jul;154(5):1094-103. [6]. F Schmidlin, et al. Protease-activated receptors: how proteases signal to cells. Curr Opin Pharmacol. 2001 Dec;1(6):575-82.

Melting Point	115°C
Molecular Formula	C₆H₁₅O₁₂P₃
Molecular Weight	372.1
Appearance	lyophilized powder
Storage condition	−20°C
Stability	Stable. Incompatible with strong oxidizing agents.
Water Solubility	Reconstitute in aqueous buffer \| Soluble in water (10 mg/ml), phosphate buffers (10 mg/ml), and balanced salt solutions (1 mg/ml).

Symbol	GHS07, GHS08
Signal Word	Danger
Hazard Statements	H315-H319-H334-H335
Precautionary Statements	P261-P305 + P351 + P338-P342 + P311
Personal Protective Equipment	dust mask type N95 (US);Eyeshields;Faceshields;Gloves
Hazard Codes	Xn,B
Risk Phrases	36/37/38-42-42/43
Safety Phrases	22-24-26-36/37-45-23
RIDADR	NONH for all modes of transport
WGK Germany	2
RTECS	GC3050000

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