Fructose 2,6-biphosphate

Modify Date: 2024-01-03 15:58:10

Fructose 2,6-biphosphate Structure
Fructose 2,6-biphosphate structure
Common Name Fructose 2,6-biphosphate
CAS Number 79082-92-1 Molecular Weight 340.11600
Density 2.06g/cm3 Boiling Point 760.3ºC at 760 mmHg
Molecular Formula C6H14O12P2 Melting Point N/A
MSDS N/A Flash Point 413.6ºC

 Use of Fructose 2,6-biphosphate


Fructose 2,6-bisphosphate is a potent stimulator of 6-Phosphofructo-1-kinase (PFK-1) and also inhibits Fructose-1,6-Bisphosphatase.

 Names

Name β-D-fructofuranose 2,6-bisphosphate
Synonym More Synonyms

 Fructose 2,6-biphosphate Biological Activity

Description Fructose 2,6-bisphosphate is a potent stimulator of 6-Phosphofructo-1-kinase (PFK-1) and also inhibits Fructose-1,6-Bisphosphatase.
Related Catalog
Target

PFK-1, Fructose-1,6-Bisphosphatase[1]

In Vitro A concentration-dependent inhibition of fructose-1,6-Bisphosphatase by Fructose 2,6-bisphosphate is observed. Another characteristic of this inhibition is that it changes the saturation curve from hyperbolic to sigmoidal. This effect is clearly apparent at 25 pM Fructose 2,6-bisphosphate. It appears that the inhibitory actions of Fructose 2,6-bisphosphate and of AMP are synergistic. Indeed, at 10 pM fructose 1,6-bisphosphate, the addition of AMP alone causes a 35% inhibition but, it increases the inhibition by 1 pM Fructose 2,6-bisphosphate from 20% to 80%[1].
Kinase Assay Fructose 1,6-bisphosphatase activity is assayed spectrophotometrically. The incubation mixture contains 100 mM KCl, 5 mM EGTA, 1 mM MgSO4, 1 mM NH4Cl, 0.2 mM NADP, 50 mM Hepes at pH 7.2, 5 μg of glucose-6-phosphate dehydrogenase per mL, and 10 μg of phosphoglucose isomerase per mL, as well as fructose 1,6-bisphosphate, AMP, and Fructose 2,6-bisphosphate at different concentrations[1].
References

[1]. Van Schaftingen E, et al. Inhibition of fructose-1,6-bisphosphatase by fructose 2,6-biphosphate. Proc Natl Acad Sci U S A. 1981 May;78(5):2861-3.

 Chemical & Physical Properties

Density 2.06g/cm3
Boiling Point 760.3ºC at 760 mmHg
Molecular Formula C6H14O12P2
Molecular Weight 340.11600
Flash Point 413.6ºC
Exact Mass 339.99600
PSA 223.06000
Index of Refraction 1.609

 Synonyms

beta-D-fructofuranose 2,6-bisphosphate
2,6-Di-O-phosphono-β-D-fructofuranose
Fructose 2,6-biphosphate