79082-92-1

79082-92-1 structure

Name: Fructose 2,6-biphosphate
Chemical Name: β-D-fructofuranose 2,6-bisphosphate
CAS Number: 79082-92-1
Molecular Formula: C₆H₁₄O₁₂P₂
Molecular Weight: 340.11600
Catalog: Research Areas Metabolic Disease
Create Date: 2016-08-21 00:34:12
Modify Date: 2024-01-03 15:58:10
Fructose 2,6-bisphosphate is a potent stimulator of 6-Phosphofructo-1-kinase (PFK-1) and also inhibits Fructose-1,6-Bisphosphatase.

Name	β-D-fructofuranose 2,6-bisphosphate
Synonyms	beta-D-fructofuranose 2,6-bisphosphate 2,6-Di-O-phosphono-β-D-fructofuranose Fructose 2,6-biphosphate

Description	Fructose 2,6-bisphosphate is a potent stimulator of 6-Phosphofructo-1-kinase (PFK-1) and also inhibits Fructose-1,6-Bisphosphatase.
Related Catalog	Signaling Pathways >> Others >> Others Research Areas >> Metabolic Disease
Target	PFK-1, Fructose-1,6-Bisphosphatase[1]
In Vitro	A concentration-dependent inhibition of fructose-1,6-Bisphosphatase by Fructose 2,6-bisphosphate is observed. Another characteristic of this inhibition is that it changes the saturation curve from hyperbolic to sigmoidal. This effect is clearly apparent at 25 pM Fructose 2,6-bisphosphate. It appears that the inhibitory actions of Fructose 2,6-bisphosphate and of AMP are synergistic. Indeed, at 10 pM fructose 1,6-bisphosphate, the addition of AMP alone causes a 35% inhibition but, it increases the inhibition by 1 pM Fructose 2,6-bisphosphate from 20% to 80%[1].
Kinase Assay	Fructose 1,6-bisphosphatase activity is assayed spectrophotometrically. The incubation mixture contains 100 mM KCl, 5 mM EGTA, 1 mM MgSO4, 1 mM NH4Cl, 0.2 mM NADP, 50 mM Hepes at pH 7.2, 5 μg of glucose-6-phosphate dehydrogenase per mL, and 10 μg of phosphoglucose isomerase per mL, as well as fructose 1,6-bisphosphate, AMP, and Fructose 2,6-bisphosphate at different concentrations[1].
References	[1]. Van Schaftingen E, et al. Inhibition of fructose-1,6-bisphosphatase by fructose 2,6-biphosphate. Proc Natl Acad Sci U S A. 1981 May;78(5):2861-3.