Fructose 2,6-biphosphate

Fructose 2,6-bisphosphate is a potent stimulator of 6-Phosphofructo-1-kinase (PFK-1) and also inhibits Fructose-1,6-Bisphosphatase.

Signaling Pathways >> Others >> Others

Research Areas >> Metabolic Disease

PFK-1, Fructose-1,6-Bisphosphatase[1]

A concentration-dependent inhibition of fructose-1,6-Bisphosphatase by Fructose 2,6-bisphosphate is observed. Another characteristic of this inhibition is that it changes the saturation curve from hyperbolic to sigmoidal. This effect is clearly apparent at 25 pM Fructose 2,6-bisphosphate. It appears that the inhibitory actions of Fructose 2,6-bisphosphate and of AMP are synergistic. Indeed, at 10 pM fructose 1,6-bisphosphate, the addition of AMP alone causes a 35% inhibition but, it increases the inhibition by 1 pM Fructose 2,6-bisphosphate from 20% to 80%[1].

Fructose 1,6-bisphosphatase activity is assayed spectrophotometrically. The incubation mixture contains 100 mM KCl, 5 mM EGTA, 1 mM MgSO4, 1 mM NH4Cl, 0.2 mM NADP, 50 mM Hepes at pH 7.2, 5 μg of glucose-6-phosphate dehydrogenase per mL, and 10 μg of phosphoglucose isomerase per mL, as well as fructose 1,6-bisphosphate, AMP, and Fructose 2,6-bisphosphate at different concentrations[1].

[1]. Van Schaftingen E, et al. Inhibition of fructose-1,6-bisphosphatase by fructose 2,6-biphosphate. Proc Natl Acad Sci U S A. 1981 May;78(5):2861-3.

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