Corynoxeine
Modify Date: 2024-01-08 15:50:31
Corynoxeine structure
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Common Name | Corynoxeine | ||
|---|---|---|---|---|
| CAS Number | 630-94-4 | Molecular Weight | 382.453 | |
| Density | 1.3±0.1 g/cm3 | Boiling Point | 562.7±50.0 °C at 760 mmHg | |
| Molecular Formula | C22H26N2O4 | Melting Point | 210℃ | |
| MSDS | N/A | Flash Point | 294.1±30.1 °C | |
Use of CorynoxeineCorynoxeine, isolated from the hook of Uncaria rhynchophylla, is a potent ERK1/ERK2 inhibitor of key PDGF-BB-induced vascular smooth muscle cells (VSMCs) proliferation. |
| Name | (±)-corynoxeine |
|---|---|
| Synonym | More Synonyms |
| Description | Corynoxeine, isolated from the hook of Uncaria rhynchophylla, is a potent ERK1/ERK2 inhibitor of key PDGF-BB-induced vascular smooth muscle cells (VSMCs) proliferation. |
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| Related Catalog | |
| Target |
ERK1 ERK2 |
| In Vitro | Corynoxeine is able to inhibit the PDGF-BB-stimulated proliferation of VSMCs through downregulation of PDGF-BB-induced ERK1/2 activation. Pre-incubation of VSMCs with Corynoxeine significantly inhibits PDGF-BB-induced extracellular signal-regulated kinase 1/2 (ERK1/2) activation, whereas Corynoxeine has no effects on mitogen-activated protein kinase (MAPK/ERK)-activating kinase 1 and 2 (MEK1/2), Akt, or phospholipase C (PLC) γ 1 activation or on PDGF receptor beta (PDGF-Rβ) phosphorylation. Corynoxeine inhibits PDGF-BB-induced ERK1/2 activation, in the same concentration range that inhibits VSMC proliferation and DNA synthesis. Corynoxeine inhibits VSMC numbers in response to PDGF-BB with 50% inhibitory concentrations (IC50) of 13.7 μM. Corynoxeine inhibits DNA synthesis in response to PDGF-BB (24 h) with IC50 of 9.2 μM. Pre-treatment of VSMCs with Corynoxeine (5-50 μM) for 24 h results in significant decreases in cell number without any cytotoxicity; the inhibition percentages are 25.0±12.5, 63.0±27.5 and 88.0±12.5% at 5, 20 and 50 μM, respectively. Corynoxeine also significantly inhibits the 50 ng/mL PDGF-BB-induced DNA synthesis of VSMCs in a concentration-dependent manner without any cytotoxicity; the inhibitions are 32.8±11.0, 51.8±8.0 and 76.9±7.4% at concentrations of 5, 20 and 50 μM, respectively[1]. |
| Cell Assay | Cell proliferation and DNA synthesis are measured. For cell counting, VSMCs are seeded in 12-well culture plates at 5-6×104 cells/mL and cultured in DMEM with 10% FBS at 37°C for 24 h. Under these conditions, the cells reach 70% confluence. The medium is then replaced by serum-free medium with Corynoxeine (5-50 μM). The cells are stimulated with 50 ng/mL PDGF-BB, then trypsinized with trypsin-EDTA and counted using a hemocytometer under a microscope. For [3H]-thymidine incorporation experiments, VSMCs are seeded in 24-well culture plates 5000 cells/well and then allowed to grow for 3-4 d in DMEM, and 2 μCi/mL of [3H]-thymidine are added to the medium. The reactions are terminated after 4 h by aspirating the medium and subjecting the cultures to sequential washes on ice with PBS containing 10% trichloroacetic acid and ethanol/ether (1 : 1, v/v). Acid-insoluble [3H]-thymidine is extracted into 250 μL of 0.5 M NaOH/well; this solution is then mixed with 3ml of scintillation cocktail and quantified using a liquid scintillation counter[1]. |
| References |
| Density | 1.3±0.1 g/cm3 |
|---|---|
| Boiling Point | 562.7±50.0 °C at 760 mmHg |
| Melting Point | 210℃ |
| Molecular Formula | C22H26N2O4 |
| Molecular Weight | 382.453 |
| Flash Point | 294.1±30.1 °C |
| Exact Mass | 382.189270 |
| PSA | 67.87000 |
| LogP | 3.07 |
| Vapour Pressure | 0.0±1.5 mmHg at 25°C |
| Index of Refraction | 1.606 |
| Hazard Codes | T+ |
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Corynoxeine CAS#:630-94-4 |
| Literature: European Journal of Organic Chemistry, , # 6 p. 1100 - 1106 |
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Corynoxeine CAS#:630-94-4 |
| Literature: European Journal of Organic Chemistry, , # 6 p. 1100 - 1106 |
|
~%
Corynoxeine CAS#:630-94-4 |
| Literature: European Journal of Organic Chemistry, , # 6 p. 1100 - 1106 |
|
~%
Corynoxeine CAS#:630-94-4 |
| Literature: European Journal of Organic Chemistry, , # 6 p. 1100 - 1106 |
| Precursor 2 | |
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| DownStream 2 | |
CAS#:76-66-4
CAS#:17391-18-3| (+/-)-corynoxeine |
| Corynoxeine |
| ()-corynoxeine |
| Spiro[3H-indole-3,1'(5'H)-indolizine]-7'-acetic acid, 6'-ethenyl-1,2,2',3',6',7',8',8'a-octahydro-α-(methoxymethylene)-2-oxo-, methyl ester, (αE,3R,6'R,7'S,8'aS)- |
| ISOCORYNOXEINE |
| Methyl (7β,16E,20α)-17-methoxy-2-oxocorynoxa-16,18-dien-16-carboxylate |
- Shanghai Jizhi Biochemical Technology Co., Ltd
- China
- Product Name: Corynoxeine
- Price: ￥1378.0/20mg
- Purity: 98.0%
- Stocking Period: 1 Day
- Contact: Liu jia
- DC Chemicals Limited
- China
- Product Name: Corynoxeine
- Price: $280.0/20mg
- Purity: 98.0%
- Stocking Period: 3 Day
- Contact: Tony Cao
- Dayang Chem (Hangzhou) Co., Ltd.
- China
- Product Name: Corynoxeine
- Price: $Inquiry/100g $Inquiry/1kg $Inquiry/100kg $Inquiry/1000kg
- Purity: 98.0%
- Stocking Period: Inquiry
- Contact: Ms Wang
- Zhejiang Hangyu API Co., Ltd
- China
- Product Name: corynoxeine
- Price: ￥Inquiry/1kg
- Purity: 99.5%
- Stocking Period: Inquiry
- Contact: Vinnie
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Price: $165/10mM*1mLinDMSO
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