Ponceau S

Modify Date: 2025-08-21 16:11:02

Ponceau S Structure
Ponceau S structure
Common Name Ponceau S
CAS Number 6226-79-5 Molecular Weight 760.569
Density 1.01 g/mL at 20 °C Boiling Point N/A
Molecular Formula C22H12N4Na4O13S4 Melting Point N/A
MSDS Chinese USA Flash Point N/A
Symbol GHS07
GHS07
Signal Word Warning

 Use of Ponceau S


Ponceau S is the most commonly used stain for Western blotting. Ponceau S is applied as an acidic aqueous solution, and it is compatible with antibody-antigen binding, and stains the proteins on the membrane red[1][2].

 Names

Name Ponceau S
Synonym More Synonyms

 Ponceau S Biological Activity

Description Ponceau S is the most commonly used stain for Western blotting. Ponceau S is applied as an acidic aqueous solution, and it is compatible with antibody-antigen binding, and stains the proteins on the membrane red[1][2].
Related Catalog
References

[1]. Sander H, et, al. Ponceau S waste: Ponceau S staining for total protein normalization. Anal Biochem. 2019 Jun 15; 575: 44-53.

[2]. Litovchick. Staining the Blot for Total Protein with Ponceau S. Cold Spring Harb Protoc. 2020 Mar 2; 2020(3): 098459.

 Chemical & Physical Properties

Density 1.01 g/mL at 20 °C
Molecular Formula C22H12N4Na4O13S4
Molecular Weight 760.569
Exact Mass 759.887451
PSA 331.99000
LogP 7.31580
Index of Refraction n20/D 1.338
InChIKey KQHKSGRIBYJYFX-UHFFFAOYSA-J
SMILES O=S(=O)([O-])c1ccc(N=Nc2ccc(N=Nc3c(O)c(S(=O)(=O)[O-])cc4cc(S(=O)(=O)[O-])ccc34)c(S(=O)(=O)[O-])c2)cc1.[Na+].[Na+].[Na+].[Na+]
Storage condition Store at RT.
Water Solubility 10 g/L

 Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :
QJ6600000
CHEMICAL NAME :
2,7-Naphthalenedisulfonic acid, 3-hydroxy-4-((2-sulfo-4-((4-sulfophenyl)azo)phenyl)az o)-, tetrasodium salt
CAS REGISTRY NUMBER :
6226-79-5
LAST UPDATED :
199701
DATA ITEMS CITED :
5
MOLECULAR FORMULA :
C22-H16-N4-O13-S4.4Na
MOLECULAR WEIGHT :
764.62

 Safety Information

Symbol GHS07
GHS07
Signal Word Warning
Hazard Statements H315-H319-H335
Precautionary Statements P280-P305 + P351 + P338-P337 + P313
Personal Protective Equipment dust mask type N95 (US);Eyeshields;Gloves
Hazard Codes Xi:Irritant;
Risk Phrases R36/37/38
Safety Phrases S26-S36-S37/39
RIDADR NONH for all modes of transport
WGK Germany 3
RTECS QJ6600000
HS Code 32041200

 Customs

HS Code 32041200

 Articles50

More Articles
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Many neuronal cytosolic and nuclear proteins are post-translationally modified by the reversible addition of O-linked N-acetylglucosamine (O-GlcNAc) on serines and threonines. The cellular functions o...

Antioxidant Supplementation Modulates Age-Related Placental Bed Morphology and Reproductive Outcome in Mice.

Biol. Reprod. 93 , 56, (2015)

The number of women who delay their first childbirth is increasing. This demographic shift is an important health issue because advanced maternal age is a risk factor for reproductive capacity loss an...

Detection of proteins on blot membranes.

Curr. Protoc. Protein Sci. Chapter 10 , Unit 10.8, (2001)

Staining of blot transfer membranes permits visualization of proteins and allows the extent of transfer to be monitored. In the protocols described in this unit, proteins are stained after electroblot...

 Synonyms

PONCEAU S EXTRA
Tetrasodium 3-hydroxy-4-[(E)-{2-sulfonato-4-[(E)-(4-sulfonatophenyl)diazenyl]phenyl}diazenyl]naphthalene-2,7-disulfonate
Tetrasodium 3-hydroxy-4-[(E)-{2-sulfonato-4-[(E)-(4-sulfonatophenyl)diazenyl]phenyl}diazenyl]-2,7-naphthalenedisulfonate
EINECS 228-319-2
3-Hydroxy-4-[2-sulfo-4-(4-sulfophenylazo)phenylazo]-2,7-naphthalenedisulfonic Acid Tetrasodium Salt
c.i.acidred112
2,7-Naphthalenedisulfonic acid, 3-hydroxy-4-((2-sulfo-4-((4-sulfophenyl)azo)phenyl)azo)-, tetrasodium salt
Ponceau Red S
Acid Red 112
ATX PONCEAU S RED
2,7-Naphthalenedisulfonic acid, 3-hydroxy-4-[(E)-2-[2-sulfo-4-[(E)-2-(4-sulfophenyl)diazenyl]phenyl]diazenyl]-, sodium salt (1:4)
FAST PONCEAU 2B
MFCD00003892
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