Ponceau S

Names

[ Name ]:
Ponceau S

[Synonym ]:
PONCEAU S EXTRA
Tetrasodium 3-hydroxy-4-[(E)-{2-sulfonato-4-[(E)-(4-sulfonatophenyl)diazenyl]phenyl}diazenyl]naphthalene-2,7-disulfonate
Tetrasodium 3-hydroxy-4-[(E)-{2-sulfonato-4-[(E)-(4-sulfonatophenyl)diazenyl]phenyl}diazenyl]-2,7-naphthalenedisulfonate
EINECS 228-319-2
3-Hydroxy-4-[2-sulfo-4-(4-sulfophenylazo)phenylazo]-2,7-naphthalenedisulfonic Acid Tetrasodium Salt
c.i.acidred112
2,7-Naphthalenedisulfonic acid, 3-hydroxy-4-((2-sulfo-4-((4-sulfophenyl)azo)phenyl)azo)-, tetrasodium salt
Ponceau Red S
Acid Red 112
ATX PONCEAU S RED
2,7-Naphthalenedisulfonic acid, 3-hydroxy-4-[(E)-2-[2-sulfo-4-[(E)-2-(4-sulfophenyl)diazenyl]phenyl]diazenyl]-, sodium salt (1:4)
FAST PONCEAU 2B
MFCD00003892

Biological Activity

[Description]:

Ponceau S is the most commonly used stain for Western blotting. Ponceau S is applied as an acidic aqueous solution, and it is compatible with antibody-antigen binding, and stains the proteins on the membrane red[1][2].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[References]

[1]. Sander H, et, al. Ponceau S waste: Ponceau S staining for total protein normalization. Anal Biochem. 2019 Jun 15; 575: 44-53.

[2]. Litovchick. Staining the Blot for Total Protein with Ponceau S. Cold Spring Harb Protoc. 2020 Mar 2; 2020(3): 098459.

Chemical & Physical Properties

[ Density]:
1.01 g/mL at 20 °C

[ Molecular Formula ]:
C₂₂H₁₂N₄Na₄O₁₃S₄

[ Molecular Weight ]:
760.569

[ Exact Mass ]:
759.887451

[ PSA ]:
331.99000

[ LogP ]:
7.31580

[ Index of Refraction ]:
n20/D 1.338

[ Storage condition ]:
Store at RT.

[ Water Solubility ]:
10 g/L

MSDS

Click to get detail MSDS

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: QJ6600000

CHEMICAL NAME :: 2,7-Naphthalenedisulfonic acid, 3-hydroxy-4-((2-sulfo-4-((4-sulfophenyl)azo)phenyl)az o)-, tetrasodium salt

CAS REGISTRY NUMBER :: 6226-79-5

LAST UPDATED :: 199701

DATA ITEMS CITED :: 5

MOLECULAR FORMULA :: C22-H16-N4-O13-S4.4Na

MOLECULAR WEIGHT :: 764.62

Safety Information

[ Symbol ]:

GHS07

[ Signal Word ]:
Warning

[ Hazard Statements ]:
H315-H319-H335

[ Precautionary Statements ]:
P280-P305 + P351 + P338-P337 + P313

[ Personal Protective Equipment ]:
dust mask type N95 (US);Eyeshields;Gloves

[ Hazard Codes ]:
Xi:Irritant;

[ Risk Phrases ]:
R36/37/38

[ Safety Phrases ]:
S26-S36-S37/39

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

[ RTECS ]:
QJ6600000

[ HS Code ]:
32041200

Customs

[ HS Code ]: 32041200

Articles

O-GLcNAc post-translational modifications regulate the entry of neurons into an axon branching program.

Dev. Neurobiol. 69(2-3) , 162-73, (2009)

Many neuronal cytosolic and nuclear proteins are post-translationally modified by the reversible addition of O-linked N-acetylglucosamine (O-GlcNAc) on serines and threonines. The cellular functions o...

Antioxidant Supplementation Modulates Age-Related Placental Bed Morphology and Reproductive Outcome in Mice.

Biol. Reprod. 93 , 56, (2015)

The number of women who delay their first childbirth is increasing. This demographic shift is an important health issue because advanced maternal age is a risk factor for reproductive capacity loss an...

Detection of proteins on blot membranes.

Curr. Protoc. Protein Sci. Chapter 10 , Unit 10.8, (2001)

Staining of blot transfer membranes permits visualization of proteins and allows the extent of transfer to be monitored. In the protocols described in this unit, proteins are stained after electroblot...

Related Compounds

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