6-Carboxyfluorescein

Modify Date: 2024-01-03 16:21:12

6-Carboxyfluorescein Structure
6-Carboxyfluorescein structure
Common Name 6-Carboxyfluorescein
CAS Number 3301-79-9 Molecular Weight 376.316
Density 1.7±0.1 g/cm3 Boiling Point 736.4±60.0 °C at 760 mmHg
Molecular Formula C21H12O7 Melting Point >300 °C(lit.)
MSDS Chinese USA Flash Point 271.0±26.4 °C

 Use of 6-Carboxyfluorescein


The single isomer, 6-FAM, contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility, fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser.

 Names

Name 6-carboxyfluorescein
Synonym More Synonyms

 6-Carboxyfluorescein Biological Activity

Description The single isomer, 6-FAM, contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility, fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser.
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 Chemical & Physical Properties

Density 1.7±0.1 g/cm3
Boiling Point 736.4±60.0 °C at 760 mmHg
Melting Point >300 °C(lit.)
Molecular Formula C21H12O7
Molecular Weight 376.316
Flash Point 271.0±26.4 °C
Exact Mass 376.058289
PSA 113.29000
LogP 2.66
Vapour Pressure 0.0±2.5 mmHg at 25°C
Index of Refraction 1.816
Storage condition 2-8°C
Water Solubility DMSO: soluble

 Safety Information

Personal Protective Equipment dust mask type N95 (US);Eyeshields;Gloves
Hazard Codes Xi: Irritant;
Risk Phrases R36/37/38
Safety Phrases S26-S36/37
RIDADR NONH for all modes of transport
WGK Germany 3
HS Code 2915900090

 Synthetic Route

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6-Carboxyfluorescein Structure

6-Carboxyfluorescein

CAS#:3301-79-9

Literature: Russian Journal of General Chemistry, , vol. 65, # 3.2 p. 454 - 456 Zhurnal Obshchei Khimii, , vol. 65, # 3 p. 511 - 513

 Precursor & DownStream

Precursor  2

DownStream  1

 Customs

HS Code 2915900090
Summary 2915900090 other saturated acyclic monocarboxylic acids and their anhydrides, halides, peroxides and peroxyacids; their halogenated, sulphonated, nitrated or nitrosated derivatives VAT:17.0% Tax rebate rate:9.0% Supervision conditions:AB(certificate of inspection for goods inward,certificate of inspection for goods outward) MFN tariff:5.5% General tariff:30.0%

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 Synonyms

6-carboxy-fluorescein
6-Carboxyfluorescein
3',6'-Dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-6-carboxylic acid
Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-6-carboxylic acid, 3',6'-dihydroxy-3-oxo-
6-FAM
6-FAM Hydrate
Carboxyfluorescein
MFCD00036873
6-FAM,6-CF
6-FAM FLUOS
EINECS 276-331-1
6-FAM,FLUOS
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